Campos-Pérez J J, Ellis A E, Secombes C J
FRS Marine Laboratory, Aberdeen, United Kingdom.
Dis Aquat Organ. 2000 Nov 14;43(2):109-15. doi: 10.3354/dao043109.
The inhibitory effect of the nitric oxide (NO) donor S-nitroso-acetyl-penicillamine (SNAP) and the NO and O2- donor 3-morpholino-sydnonimine hydrochloride (SIN-1) was tested in a cell-free assay. Strains of the bacterial fish pathogens Aeromonas salmonicida, Renibacterium salmoninarum and Yersinia ruckeri were exposed to different concentrations of the NO donors for 24 h. The results showed that NO possesses inhibitory properties, while peroxynitrite had no effect. However, when SIN-1 was used in combination with superoxide dismutase (SOD) alone or with catalase, an inhibitory effect comparable to that caused by SNAP was seen. The implications of these results are discussed.
在无细胞试验中测试了一氧化氮（NO）供体S-亚硝基乙酰青霉胺（SNAP）以及NO和O2-供体盐酸3-吗啉代西多胺（SIN-1）的抑制作用。将细菌性鱼类病原体鲑气单胞菌、鲑肾杆菌和鲁氏耶尔森菌菌株暴露于不同浓度的NO供体中24小时。结果表明，NO具有抑制特性，而过氧亚硝酸盐则无作用。然而，当SIN-1单独与超氧化物歧化酶（SOD）或与过氧化氢酶联合使用时，可观察到与SNAP所产生的抑制作用相当的效果。讨论了这些结果的意义。
