Krahn D F, Heidelberger C
Mutat Res. 1977 Feb 1;46(1):27-44. doi: 10.1016/0165-1161(77)90108-x.
Several chemical carcinogens that require metabolic activation have been examined for their cytotoxic and mutagenic activity in Chinese hamster V79 cells. Mutagenic activity was measured as the induced frequency of 6-thioguanine-resistant colonies. Metabolic activation was provided by the 9000 g supernatant fraction of rat liver plus cofactors. The cytotoxicity and mutagenicity of aflatoxin B1 and B2, benzo(a)pyrene, 3-methylcholanthrene, 7,12-dimethylbenz(a)anthracene, dibenz(a,h)-anthracene, dibenz(a,c)anthracene, and benz(a)anthracene were examined as functions of concentration. Except for the two isomers of dibenzanthracene, the mutagenic activity in general paralleled the carcinogenic activity. An assay of this type may be useful as a prescreen for environmental chemicals that require metabolic activation.
已经在中国仓鼠V79细胞中检测了几种需要代谢活化的化学致癌物的细胞毒性和诱变活性。诱变活性通过6-硫鸟嘌呤抗性菌落的诱导频率来衡量。代谢活化由大鼠肝脏9000g上清液组分加辅助因子提供。研究了黄曲霉毒素B1和B2、苯并(a)芘、3-甲基胆蒽、7,12-二甲基苯并(a)蒽、二苯并(a,h)蒽、二苯并(a,c)蒽和苯并(a)蒽的细胞毒性和诱变性与浓度的关系。除二苯并蒽的两种异构体外，诱变活性总体上与致癌活性平行。这种类型的检测可作为需要代谢活化的环境化学物质的预筛选方法。
