Groettrup M, Ungewiss K, Azogui O, Palacios R, Owen M J, Hayday A C, von Boehmer H
Basel Institute for Immunology, Switzerland.
Cell. 1993 Oct 22;75(2):283-94. doi: 10.1016/0092-8674(93)80070-u.
We describe a novel signal-transducing protein complex, which consists of the T cell receptor (TCR) beta chain that is disulfide linked to a 33 kd glycoprotein and noncovalently associated with proteins of the CD3 complex on the surface of the pre-T cell line SCB.29. This 33 kd glycoprotein, provisionally designated gp33, represents neither of the known TCR chains and has escaped previous detection because it labels poorly by surface iodination. This glycoprotein is absent from the surface of mature T cell lines. A TCR beta complex with identical molecular masses before and after reduction can be immunoprecipitated from surface-iodinated large thymocytes of TCR alpha-deficient mice. The novel gp33-TCR beta complex may be entirely or partly responsible for control of early T cell development exerted by the TCR beta protein.
我们描述了一种新型的信号转导蛋白复合物，它由与33kd糖蛋白通过二硫键相连的T细胞受体（TCR）β链组成，并与前T细胞系SCB.29表面的CD3复合物蛋白非共价结合。这种33kd糖蛋白，暂命名为gp33，既不代表已知的TCR链，且由于其表面碘化标记效果不佳而此前未被检测到。成熟T细胞系表面不存在这种糖蛋白。从TCRα缺陷小鼠的表面碘化大胸腺细胞中可免疫沉淀出还原前后分子量相同的TCRβ复合物。这种新型的gp33-TCRβ复合物可能全部或部分负责TCRβ蛋白对早期T细胞发育的调控。
