Kang D W, Ohkawa S, Difabio S, Merrill K W, Fujihashi K, Yamamoto M, Miller C J, Marthas M, McGhee J R, Eldridge J H
Department of Microbiology, University of Alabama at Birmingham 35294.
Cell Immunol. 1993 Oct 15;151(2):379-91. doi: 10.1006/cimm.1993.1247.
This study has assessed T cell subsets according to the expression of CD4 and CD8 and the isotype of surface Ig+ (sIg+) B cells and plasma cells in mononuclear cells (MC) isolated from mucosa-associated tissues of rhesus macaques in comparison to lymphoid cells from systemic tissues, i.e., spleen and peripheral lymph nodes (PLN). Using enzymatic and/or mechanical dissociation methods, mononuclear cells were isolated from lamina propria (LP) of the small (jejunum and ileum) and large (cecum) intestines, parotid glands (PG), and mesenteric lymph nodes (MLN). Approximately equal numbers of CD4+ and CD8+ T cells occurred in mucosa-associated tissues (CD4:CD8 ratios of 0.9-1.2), while slightly higher numbers of CD8+ T cells were seen in spleen and PLN (CD4:CD8 ratios of 0.6-0.9). When the isotypes of sIg+ B cells were assessed in MC isolated from mucosa-associated tissues, the highest frequency of sIgA+ B cells was seen in MLN, which may represent B cells which had recently migrated from IgA inductive sites. However, IgA effector tissues, such as intestinal LP and PG, contained frequencies of sIgA+ B cells lower than those of the MLN. When Ig-producing cells were examined, 50-90% of Ig-secreting cells were of the IgA class. This suggests that sIgA+ B cells differentiate into IgA plasma cells at higher rates in the mucosal effector tissues. Plasma cells of IgM isotype were also found in significant numbers in the LP of the intestine and PG, while IgG plasma cells were most prevalent in spleen and PLN. Taken together, mucosa-associated tissues of rhesus macaques are characterized by higher numbers of CD4+ T cells and Ig-secreting plasma cells of IgA and IgM isotypes than those of systemic lymphoid tissues.
本研究根据恒河猴黏膜相关组织分离出的单核细胞（MC）中CD4和CD8的表达以及表面Ig +（sIg +）B细胞和浆细胞的同种型，评估了T细胞亚群，并与来自全身组织（即脾脏和外周淋巴结（PLN））的淋巴细胞进行了比较。使用酶解和/或机械解离方法，从小肠（空肠和回肠）和大肠（盲肠）的固有层（LP）、腮腺（PG）和肠系膜淋巴结（MLN）中分离出单核细胞。黏膜相关组织中CD4 +和CD8 + T细胞数量大致相等（CD4：CD8比率为0.9 - 1.2），而脾脏和PLN中CD8 + T细胞数量略多（CD4：CD8比率为0.6 - 0.9）。当评估从黏膜相关组织分离的MC中sIg + B细胞的同种型时，在MLN中观察到sIgA + B细胞的频率最高，这可能代表最近从IgA诱导部位迁移来的B细胞。然而，IgA效应组织，如肠道LP和PG，sIgA + B细胞的频率低于MLN。当检查产生Ig的细胞时，50 - 90%的Ig分泌细胞为IgA类。这表明sIgA + B细胞在黏膜效应组织中以更高的速率分化为IgA浆细胞。在肠道LP和PG中也发现了大量IgM同种型的浆细胞，而IgG浆细胞在脾脏和PLN中最为普遍。综上所述，恒河猴黏膜相关组织的特征是CD4 + T细胞以及IgA和IgM同种型的分泌Ig的浆细胞数量高于全身淋巴组织。
