Ishihara Y, Ohkubo T, Shimazaki H, el Borai N, Takano J, Yamamura M
Department of Molecular Life Science I, Tokai University School of Medicine, Kanagawa, Japan.
Cell Biochem Funct. 1993 Sep;11(3):221-4. doi: 10.1002/cbf.290110310.
A new continuous column culture system for adherent cells was developed using beads. The beads were packed in a column and an appropriate medium was continuously passed through. The whole system was kept under closed conditions. L cells and C6 cells were cultured by this new system. The number of cells increased linearly up to 16 days and reached a maximum at around 18 days. As the heat production remained constant for 16 days, it can be concluded that cells grown in this system had identical characteristics. The final concentration of cells reached was 1.0 x 10(8) ml-1. The cells could grow both in the upward and the downward direction. Advantages of this system are: (1) Cells can be recovered in their adherent form on the beads; (2) cells can easily be collected from the column by trypsinization, and (3) cells remaining in the column after trypsinization can grow again.
利用珠子开发了一种用于贴壁细胞的新型连续柱培养系统。珠子被填充在柱中，合适的培养基连续通过。整个系统保持在封闭条件下。用这种新系统培养了L细胞和C6细胞。细胞数量在长达16天的时间内呈线性增加，并在大约18天达到最大值。由于产热在16天内保持恒定，可以得出结论，在该系统中生长的细胞具有相同的特性。最终达到的细胞浓度为1.0×10(8)个/毫升。细胞可以向上和向下生长。该系统的优点是：(1) 细胞可以以贴壁形式在珠子上回收；(2) 细胞可以通过胰蛋白酶消化轻松地从柱中收集，并且 (3) 胰蛋白酶消化后留在柱中的细胞可以再次生长。
