Duan Y F, Song C C, Shou G C, Zhu H, Shi J F, Fu J K, Zhang Q Y
Institute of Parasitic Diseases, Zhejiang Academy of Medical Sciences, Hangzhou.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1993;11(1):45-9.
The purpose of the present study was to observe the survival and development of Clonorchis sinensis metacercariae in their final hosts after Co-60 gamma irradiation exerting on both metacercariae isolated or in fish. Guinea pigs or albino rats were orally infected by gavage. Bio-assay, fecal examination for ova and dissection of infected animals were used for the estimation of minimum effective dose of gamma irradiation to control infectivity of Clonorchis sinensis metacercariae. Results showed that the minimum effective irradiation dose for isolated metacercariae was 0.05 kGy. The LD50 of the irradiation dose for metacercariae in fish was 0.05 kGy, and the minimum effective dose was 0.15 kGy. No significant difference in radiation susceptibility to Co-60 gamma irradiation was found among Clonorchis sinensis metacercariae in fishes collected at different localities in the northern, middle or southern parts of China. The present finding suggests that irradiation of the fish at a dose of 0.15 kGy could control the infectivity of Clonorchis sinensis metacercariae and thus be adopted as a control measure in preventive medicine.
本研究的目的是观察华支睾吸虫囊蚴在离体或鱼体中经钴-60γ射线照射后在其终末宿主体内的存活及发育情况。通过灌胃法对豚鼠或白化大鼠进行经口感染。采用生物测定、粪便虫卵检查及对感染动物进行解剖等方法，来评估γ射线控制华支睾吸虫囊蚴感染性的最小有效剂量。结果显示，离体囊蚴的最小有效照射剂量为0.05千戈瑞。鱼体中囊蚴的照射剂量半数致死量（LD50）为0.05千戈瑞，最小有效剂量为0.15千戈瑞。在中国北部、中部或南部不同地点采集的鱼体中的华支睾吸虫囊蚴，对钴-60γ射线的辐射敏感性未发现显著差异。本研究结果表明，以0.15千戈瑞的剂量照射鱼体可控制华支睾吸虫囊蚴的感染性，因此可作为预防医学中的一项控制措施采用。
