Schellinck H M, Smyth C, Brown R, Wilkinson M
Department of Psychology, Dalhousie University, Halifax, N.S., Canada.
Brain Res Dev Brain Res. 1993 Jul 16;74(1):138-41. doi: 10.1016/0165-3806(93)90094-q.
Exposure to the urine or soiled bedding odors of novel adult males is known to accelerate puberty in juvenile female mice. To determine what part of the olfactory system is activated by these odors, the expression of c-fos in the main olfactory bulb (MOB) and the accessory olfactory bulb (AOB) of juvenile female mice was examined after their exposure to male-soiled bedding, peppermint odor or their own bedding. Fos-like immunoreactivity was found throughout the AOB of the juvenile female mice exposed to the bedding odors of adult males for 3 h. In contrast, dense staining was found in the granular cell layer of the MOB of mice exposed to peppermint odors for 3 h, whereas mice exposed to their own bedding failed to show immunostaining in the AOB and only slight or no staining in the MOB. These results indicate that social odors stimulate the expression of c-fos in the AOB while non-social odors activate the MOB. This method allows the identification of individual cells activated by the different odors and will be useful in locating other areas of the brain involved in the neuroendocrine changes underlying odor-induced precocious puberty.
已知接触新奇成年雄性小鼠的尿液或弄脏的垫料气味会加速幼年雌性小鼠的青春期。为了确定嗅觉系统的哪个部分被这些气味激活，在幼年雌性小鼠接触雄性弄脏的垫料、薄荷气味或它们自己的垫料后，检测了其主嗅球（MOB）和副嗅球（AOB）中c-fos的表达。在暴露于成年雄性垫料气味3小时的幼年雌性小鼠的整个副嗅球中发现了Fos样免疫反应性。相比之下，在暴露于薄荷气味3小时的小鼠的主嗅球颗粒细胞层中发现了密集染色，而暴露于它们自己垫料的小鼠在副嗅球中未显示免疫染色，在主嗅球中仅显示轻微或无染色。这些结果表明，社交气味刺激副嗅球中c-fos的表达，而非社交气味激活主嗅球。这种方法可以识别由不同气味激活的单个细胞，并将有助于定位大脑中参与气味诱导性早熟潜在神经内分泌变化的其他区域。
