Kono T, Taniguchi S, Mizuno N, Fukuda M, Maekawa N, Hisa T, Ishii M, Otani S, Hamada T
Department of Dermatology, Osaka City University Medical School, Japan.
Clin Exp Dermatol. 1993 Jul;18(4):312-7. doi: 10.1111/j.1365-2230.1993.tb02205.x.
The activity and gene expression of ornithine decarboxylase (ODC, an indicator of tumour promotion) were induced by the phorbol ester tumour promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), in mouse skin. In the present study, the effect of colchicine, a microtubule-disrupting agent, on ODC activity and its gene expression were investigated. On administration of colchicine (100 micrograms) intraperitoneally 1.5 h before TPA treatment, ODC activity and ODC mRNA levels stimulated by TPA were suppressed to about 52 and 64%, respectively. These results suggest the involvement of a microtubule or colchicine-sensitive substrate in the signal transduction system for gene expression.
佛波酯肿瘤促进剂12 - O -十四酰佛波醇-13 -乙酸酯（TPA）可诱导小鼠皮肤中鸟氨酸脱羧酶（ODC，一种肿瘤促进指标）的活性和基因表达。在本研究中，研究了微管破坏剂秋水仙碱对ODC活性及其基因表达的影响。在TPA处理前1.5小时腹腔注射秋水仙碱（100微克），TPA刺激的ODC活性和ODC mRNA水平分别被抑制至约52%和64%。这些结果表明微管或秋水仙碱敏感底物参与了基因表达的信号转导系统。
