转化生长因子β1在HIV-1感染中淋巴细胞增殖反应降低方面缺乏作用。

Lack of role of TGF-beta 1 in decreased lymphoproliferative response in HIV-1 infection.

作者信息

Chopra R K, Raj N B, Scally J P, Cappuccio W R, Nagel J E, Saah A J, Margolick J B

机构信息

Department of Environmental Health Sciences, School of Hygiene and Public Health, Baltimore, Maryland 21205.

出版信息

Clin Immunol Immunopathol. 1993 Oct;69(1):77-82. doi: 10.1006/clin.1993.1152.

DOI:10.1006/clin.1993.1152

PMID:8403544

Abstract

We investigated whether reduction of the phytohemagglutinin (PHA)-induced proliferative response of lymphocytes from HIV type-1 (HIV-1)-infected (HIV+) individuals could be explained by overproduction of transforming growth factor-beta 1 (TGF-beta 1), a strong inhibitor of T cell proliferation. PHA-stimulated PBMC from 40 HIV- and 42 HIV+ homosexual men from the Baltimore Center of the Multicenter AIDS Cohort Study (MACS) were studied using Northern blot analysis of expression of TGF-beta 1 mRNA and determining the effects of anti-TGF-beta 1 neutralizing antibody on PHA-induced proliferative responses. Compared to the HIV- donors, HIV+ donors did not show increased expression of TGF-beta 1 mRNA in unstimulated or PHA-stimulated PBMC. Furthermore, a neutralizing antibody to TGF-beta 1 did not reverse the decreased proliferative response of PBMC from HIV+ individuals to PHA or interleukin-2. These results indicate that TGF-beta 1 is not involved in T cell proliferation defects seen in HIV+ donors.

摘要

我们研究了1型人类免疫缺陷病毒（HIV-1）感染（HIV+）个体中，植物血凝素（PHA）诱导的淋巴细胞增殖反应降低是否可由转化生长因子β1（TGF-β1，一种强力T细胞增殖抑制剂）的过量产生来解释。使用TGF-β1 mRNA表达的Northern印迹分析，并确定抗TGF-β1中和抗体对PHA诱导的增殖反应的影响，对来自多中心艾滋病队列研究（MACS）巴尔的摩中心的40名HIV阴性和42名HIV阳性同性恋男性的PHA刺激的外周血单核细胞（PBMC）进行了研究。与HIV阴性供体相比，HIV阳性供体在未刺激或PHA刺激的PBMC中未显示TGF-β1 mRNA表达增加。此外，TGF-β1中和抗体并未逆转HIV阳性个体PBMC对PHA或白细胞介素-2的增殖反应降低。这些结果表明，TGF-β1不参与HIV阳性供体中所见的T细胞增殖缺陷。