Abas N, Koivunen D G, Johnson J A
Department of Physiology, University of Missouri School of Medicine, Columbia 65201.
Contraception. 1993 Jul;48(1):71-9. doi: 10.1016/0010-7824(93)90067-h.
Rats were made hypertensive by the oral ingestion of mestranol for 6 months. Carotid arteries from these hypertensive rats and from control rats were incubated in an extract of plasma from these rats, which contained 24Na and 14C-sucrose (an extracellular fluid marker). After sufficient time for equilibration, the vessels were removed and counted for 24Na; an aliquot of the incubation fluid was also counted for 24Na. At least 7 days later, after the 24Na had decayed away, the samples were counted for 14C-sucrose. By knowing the specific activity of the 24Na and the ratio of 24Na/14C in the bathing fluid, the amount of intracellular Na could be calculated for each carotid artery. The total protein content of each vessel was determined, and the results were expressed as nEq of Na per mg of vessel. The Na content of carotids from mestranol treated rats averaged 135 +/- 11 (SEM) while the carotids from the control rats averaged 125 +/- 8 nEq/mg protein. Because these values were not significantly different, this study provided no evidence that increases in arterial Na content contributed to the hypertension associated with the ingestion of mestranol in this rat model.
通过口服炔雌醇甲醚6个月使大鼠患高血压。将这些高血压大鼠和对照大鼠的颈动脉置于含有24Na和14C - 蔗糖（一种细胞外液标志物）的这些大鼠的血浆提取物中孵育。在有足够时间达到平衡后，取出血管并对24Na进行计数；孵育液的一份等分试样也对24Na进行计数。至少7天后，在24Na衰变后，对样品进行14C - 蔗糖计数。通过了解24Na的比活度和浴液中24Na/14C的比值，可以计算出每条颈动脉的细胞内Na含量。测定每条血管的总蛋白含量，结果以每毫克血管中Na的纳摩尔当量表示。炔雌醇甲醚处理的大鼠颈动脉的Na含量平均为135±11（标准误），而对照大鼠颈动脉的Na含量平均为125±8纳摩尔当量/毫克蛋白。因为这些值没有显著差异，所以本研究没有提供证据表明动脉Na含量的增加导致了该大鼠模型中与口服炔雌醇甲醚相关的高血压。
