Garagna S, Redi C A, Capanna E, Andayani N, Alfano R M, Doi P, Viale G
Dipartimento di Biologia Animale, Universitá di Pavia, Italy.
Cytogenet Cell Genet. 1993;64(3-4):247-55. doi: 10.1159/000133587.
We compared the genome distribution, chromosomal allocation, and organization of the major and minor satellite DNAs (satDNAs) in 11 species and subspecies of the genus Mus. Southern blot analysis of the major and minor satDNAs showed similar fragment profiles in all 11 species, with the exception of M. cervicolor and M. cookii for the major satDNAs and M. caroli, M. cervicolor, and M. cookii for the minor satDNAs, where these sequences could not be detected by the probes used. In situ hybridization of the major and minor satDNA probes revealed chromosome-specific allocations of these sequences with quantitative species-specific patterns. Fluorometric analysis of the organization of the satellite sequences suggested that in the M. domesticus genome satDNA sequences are clustered in tandem repeats that are longer than those present in other Mus genomes. When compared with the other Mus genomes so far studied, the domesticus genome shows the highest quantity of satDNA sequences with a long-range organization of satDNA sequences.
我们比较了小家鼠属11个物种和亚种中主要和次要卫星DNA（satDNA）的基因组分布、染色体定位及组织形式。对主要和次要satDNA的Southern杂交分析显示，除了对于主要satDNA的中缅小鼠和库氏小鼠，以及对于次要satDNA的卡罗小鼠、中缅小鼠和库氏小鼠外，其余所有11个物种的片段图谱相似，在这些物种中，所用探针未能检测到这些序列。主要和次要satDNA探针的原位杂交揭示了这些序列在染色体上的特异性定位以及物种特异性的定量模式。对卫星序列组织形式的荧光分析表明，在小家鼠基因组中，satDNA序列以串联重复的形式聚集，这些串联重复比其他小家鼠属基因组中的更长。与目前已研究的其他小家鼠属基因组相比，小家鼠基因组显示出最高数量的satDNA序列，且satDNA序列具有远距离组织形式。
