Cazaux B, Catalan J, Justy F, Escudé C, Desmarais E, Britton-Davidian J
Institut des Sciences de l'Evolution, Université Montpellier 2, cc065, Pl. E. Bataillon, 34095 Montpellier Cedex 05, France.
Chromosoma. 2013 Jun;122(3):209-20. doi: 10.1007/s00412-013-0402-4. Epub 2013 Mar 21.
The composition and orientation of the house mouse satellite DNA sequences (minor, major, TLC) were investigated by a FISH and CO-FISH approach in 11 taxa belonging to three clades of the subgenus Mus. Using a phylogenetic framework, our results highlighted two distribution patterns. The TLC satellite, the most recently discovered satellite, was present in all clades but varied quantitatively among species. This distribution supported its appearance in the ancestor of the subgenus followed by independent evolution in species of each clade. In contrast, the minor and major satellites occurred in only two clades of the subgenus indicating the simultaneous and recent amplification of these sequences. In addition, although qualitative differences in the composition and orientation of the satellite sequences were observed among the taxa, none of the features studied were unique to the house mouse and could account for the extensive chromosomal plasticity evidenced in Mus musculus domesticus.
采用荧光原位杂交（FISH）和染色体定向荧光原位杂交（CO-FISH）方法，对小家鼠亚属三个进化枝的11个分类单元的小家鼠卫星DNA序列（次要、主要、TLC）的组成和方向进行了研究。利用系统发育框架，我们的结果突出了两种分布模式。TLC卫星是最近发现的卫星，存在于所有进化枝中，但在物种间数量有所不同。这种分布支持了它在亚属祖先中出现，随后在每个进化枝的物种中独立进化。相比之下，次要和主要卫星仅出现在亚属的两个进化枝中，表明这些序列同时且近期发生了扩增。此外，尽管在分类单元之间观察到卫星序列组成和方向的定性差异，但所研究的特征没有一个是小家鼠特有的，也无法解释家鼠所显示的广泛染色体可塑性。
