Fernando M A, Pasternak J
Parasitology. 1977 Feb;74(1):19-26. doi: 10.1017/s0031182000047491.
After infection with Eimeria necatrix, chick intestinal cells undergo a profound increase in size. Herein, we describe methods for isolating and purifying infected cells containing second-genration schizonts at various times post-infection. Our purification procedures depends upon three fundamental properties of infected cells: namely (1) large size, (2) resistance to shearing and (3) decreased susceptibility to osmotic shock in hypotonic solutions. The availability of simple techniques for isolating infected whole cells should prove valuable in elucidating various intracellular aspects of infection during schizogony.
感染毒害艾美耳球虫后，雏鸡肠道细胞的大小会显著增加。在此，我们描述了在感染后不同时间分离和纯化含有第二代裂殖体的感染细胞的方法。我们的纯化程序取决于感染细胞的三个基本特性：即（1）体积大，（2）抗剪切，以及（3）在低渗溶液中对渗透压休克的敏感性降低。分离感染的完整细胞的简单技术的可用性在阐明裂殖生殖期间感染的各种细胞内方面应该是有价值的。
