Akagawa K S, Kamoshita K, Tokunaga T
Department of Cellular Immunology, National Institute of Health, Tokyo, Japan.
J Immunol. 1988 Nov 15;141(10):3383-90.
Murine alveolar macrophages (AM) were shown to have proliferative ability and to form colonies in vitro. The factors in lung-conditioned medium (CM) and L929-CM which stimulate the proliferation of AM were considered to be granulocyte-macrophage colony-stimulating factor (GM-CSF) and CSF-1, respectively, because recombinant murine (rm)GM-CSF and recombinant human (rh)CSF-1 could replace the activities of lung-CM and L929-CM, respectively. The phenotype of the cells in the colonies formed by AM incubated with rmGM-CSF or lung-CM was AM-like; more than 90% of the cells were stained by anti-asialo GM1 but not by FITC-LPS, and had AM-like morphology. Expression of Mac-1 Ag determined by M1/70HL in these cells as well as original AM was low. However, the phenotype of the cells in the colonies formed by AM incubated with rhCSF-1 or L929-CM was peritoneal macrophage (PM)-like; more than 90% of the cells were stained by FITC-LPS and M1/70HL, but not by anti-asialo GM1, and showed PM-like morphology. The cells in the colonies formed by AM incubated with rmGMCSF changed their phenotype after treatment with rhCSF-1; the percentage of cells stained by anti-asialo GM1 decreased, and that of cells stained by FITC-LPS increased. The cells in the colonies formed by AM incubated with rhCSF-1 never changed their phenotype after incubation with rmGM-CSF. In contrast to AM, more than 90% of the cells in all colonies formed by PM incubated with either rmGM-CSF, rhCSF-1, lung-CM, or L929-CM were stained by FITC-LPS but not by anti-asialo GM1. These results show that although AM and PM can proliferate, AM, in contrast to PM, are bipotential cells that can differentiate into two types of macrophages responding to distinct types of CSF, and that one of the molecular mechanisms controlling macrophage heterogeneity may be based on the type of CSF produced at distinct tissues.
已证明小鼠肺泡巨噬细胞（AM）具有增殖能力，并能在体外形成集落。肺条件培养基（CM）和L929 - CM中刺激AM增殖的因子分别被认为是粒细胞 - 巨噬细胞集落刺激因子（GM - CSF）和CSF - 1，因为重组小鼠（rm）GM - CSF和重组人（rh）CSF - 1可分别替代肺CM和L929 - CM的活性。用rmGM - CSF或肺CM培养的AM形成的集落中的细胞表型类似AM；超过90%的细胞被抗去唾液酸GM1染色，但未被FITC - LPS染色，且具有AM样形态。用M1/70HL测定这些细胞以及原始AM中Mac - 1 Ag的表达较低。然而，用rhCSF - 1或L929 - CM培养的AM形成的集落中的细胞表型类似腹腔巨噬细胞（PM）；超过90%的细胞被FITC - LPS和M1/70HL染色，但未被抗去唾液酸GM1染色，并呈现PM样形态。用rmGMCSF培养的AM形成的集落中的细胞在用rhCSF - 1处理后改变了其表型；被抗去唾液酸GM1染色的细胞百分比降低，而被FITC - LPS染色的细胞百分比增加。用rhCSF - 1培养的AM形成的集落中的细胞在用rmGM - CSF培养后从未改变其表型。与AM相反，用rmGM - CSF, rhCSF - 1,肺CM或L929 - CM培养的PM形成的所有集落中超过90%的细胞被FITC - LPS染色，但未被抗去唾液酸GM1染色。这些结果表明，虽然AM和PM都能增殖，但与PM不同，AM是双潜能细胞，可分化为对不同类型CSF作出反应的两种巨噬细胞，并且控制巨噬细胞异质性的分子机制之一可能基于不同组织产生的CSF类型。
