Dopaminergic neuronal sprouting and behavioral recovery in hemi-parkinsonian rats after implantation of amnion cells.

Cells obtained from human, monkey, or rat term amnion membrane produce an activity which, in vitro, increases process outgrowth from rat sympathetic neurons and from dopaminergic neurons of the rat ventral mesencephalon. To determine if these cells could induce sprouting of dopaminergic nerve fibers in vivo, the substantia nigra of rats was lesioned unilaterally with 6-hydroxydopamine and live-rat-term amnion cells, or killed-rat-term amnion cells were implanted into the denervated striata. A control group of rats received saline injections into the denervated striata. Rats implanted with live amnion cells had a significant decrease in turning in response to amphetamine. The lesioned and implanted striata of live-amnion-cell-implanted rats contained significantly greater areas of tyrosine hydroxylase-immunoreactive fibers than the lesioned and implanted striatum of rats in the killed-amnion-cell or saline groups. Differences in the area of tyrosine hydroxylase-immunoreactive fibers in the implanted striata or in amphetamine-induced rotation between killed amnion cell-implanted and saline-injected rats did not reach significance. Implants of live amnion cells into the striatum of a parkinsonian animal model can evoke the de novo appearance of dopaminergic fibers in the denervated striatum and behavioral recovery, most likely through a trophic mechanism.