Barile G, Di Renzo L, Lipari M, Frati L, Faggioni A
Istituto Tecnologie Biomediche, CNR, Rome, Italy.
FEBS Lett. 1993 Jun 21;324(3):319-24. doi: 10.1016/0014-5793(93)80143-i.
The present investigation shows that C3 (hemolytic inactive) as well as C3b and C3d bind Raji, a CR2-positive Burkitt lymphoma-derived cell line. Pretreatment of the cells with OKB-7 inhibited the binding of C3, whereas pretreatment with HB-5 inhibited the binding of C3b. Furthermore, the cells coated either with OKB-7 or HB-5 bound high amounts of C3d. TPA-treated cells showed binding for C3b and weak binding for C3 and C3d. Taken together, the data suggest that Raji cells may express three binding sites for C3, C3b and C3d which can be differently modulated by anti-CR2 MoAbs and TPA.
目前的研究表明，C3（溶血无活性）以及C3b和C3d可结合Raji细胞，这是一种源自CR2阳性伯基特淋巴瘤的细胞系。用OKB-7预处理细胞可抑制C3的结合，而用HB-5预处理则可抑制C3b的结合。此外，用OKB-7或HB-5包被的细胞可结合大量C3d。经佛波酯（TPA）处理的细胞显示出对C3b的结合以及对C3和C3d的弱结合。综上所述，数据表明Raji细胞可能表达C3、C3b和C3d的三个结合位点，这些位点可被抗CR2单克隆抗体和TPA以不同方式调节。
