Arbona M, de Frutos R, Tanguay R M
Departamento de Genética, Universitat de València, Spain.
Genome. 1993 Aug;36(4):694-700. doi: 10.1139/g93-093.
In this paper we report a transcriptional and translational study of the hsp83 gene of Drosophila subobscura. This gene is located at the 18C region of the J chromosome. A monoclonal antibody raised against hsp83 was used for the immunological detection of this protein by Western blotting throughout the development of D. subobscura in control and heat-shock conditions. Our results indicate that puff 18C is not only heat-shock inducible but is also expressed during normal development and its level of expression increases at the end of the prepupa period. We detected hsp83 at normal temperatures, in particular developmental stages with the exception of the larval and the beginning of prepupa formation. Hsp83 was induced by heat shock in all stages studied with the exception of 1st instar larvae. We found that temperatures in excess of 26 degrees C were sufficient to induce hsp83. In addition, at temperatures from 26 to 34 degrees C, the increase in hsp83 synthesis was accompanied by increased transcription of the hsp83 gene; this positive correlation was not observed at 37 degrees C.
在本文中，我们报告了对果蝇hsp83基因的转录和翻译研究。该基因位于J染色体的18C区域。通过免疫印迹法，使用针对hsp83产生的单克隆抗体，在对照和热休克条件下，对果蝇整个发育过程中的这种蛋白质进行免疫检测。我们的结果表明，18C疏松区不仅可被热休克诱导，而且在正常发育过程中也有表达，并且其表达水平在预蛹期结束时升高。我们在正常温度下，在除幼虫期和预蛹形成初期之外的特定发育阶段检测到了hsp83。除一龄幼虫外，在所有研究阶段热休克均可诱导hsp83。我们发现超过26摄氏度的温度足以诱导hsp83。此外，在26至34摄氏度的温度下，hsp83合成的增加伴随着hsp83基因转录的增加；在37摄氏度时未观察到这种正相关关系。
