Leonov D, Elad D
Nucleic Acids Res. 1977 Feb;4(2):319-26. doi: 10.1093/nar/4.2.319.
Ultraviolet light-induced free radical alkylation with 2-propanol or D-ribose, initiated with di-tert-butyl peroxide, of poly (G), poly (U20G), and poly(A) led to the substitution of the appropriate group for the H-8 atom of the purines and addition across the 5,6-double bond of the pyrimidines. The alkylated polynucleotides were subjected to nucleolytic digestion with several nucleases. T1-RNase digestion of poly(G) irradiated with 2-propanol gave a mixture of the modified and non-modified mononucleotides. Similarly, pancreatic RNase digestion of the irradiated poly(U20G) resulted in a mixture of the appropriate mononucleotides. A T2-RNase treatment of poly(A) irradiated with 2-propanol gave the modified Ado-21:3'-P, while T2-RNase digestion of poly(A) irradiated with D-ribose led to the cyclic modified mononucleotides, in addition to the modified mononucleotides.
用二叔丁基过氧化物引发，使聚（G）、聚（U20G）和聚（A）与2-丙醇或D-核糖进行紫外光诱导的自由基烷基化反应，导致嘌呤的H-8原子被相应基团取代，并在嘧啶的5,6-双键处加成。将烷基化的多核苷酸用几种核酸酶进行核酸水解消化。用2-丙醇辐照后的聚（G）经T1-核糖核酸酶消化，得到修饰和未修饰单核苷酸的混合物。同样，经辐照的聚（U20G）经胰核糖核酸酶消化后，产生相应单核苷酸的混合物。用2-丙醇辐照后的聚（A）经T2-核糖核酸酶处理，得到修饰的腺苷-21:3'-P，而用D-核糖辐照后的聚（A）经T2-核糖核酸酶消化，除了修饰的单核苷酸外，还产生环状修饰的单核苷酸。
