Sparks C A, Bangs P L, McNeil G P, Lawrence J B, Fey E G
Department of Cell Biology, University of Massachusetts Medical School, Worcester 01655.
Genomics. 1993 Jul;17(1):222-4. doi: 10.1006/geno.1993.1307.
A monoclonal antibody that was specific for a nuclear matrix protein was obtained and used to screen a human lambda gt11 expression library. Several partial cDNA clones were isolated and sequenced. The sequence for this protein was shown to be identical to that of NuMA, a 236-kDa nuclear mitotic spindle apparatus protein. NuMA has been recently characterized by two independent studies, and is thought to be part of a family of proteins that is required for the completion of mitosis. In this report, the chromosomal localization and copy number of the NuMA gene are analyzed using cDNA clones. High-resolution in situ hybridization reveals a single pair of signals on sister chromatids of human chromosome 11 at band q13. Stringent Southern analysis of human genomic DNA resulted in simple restriction patterns. These results together indicate that the NuMA gene is present as a single copy on human chromosome 11q13.
获得了一种对核基质蛋白具有特异性的单克隆抗体，并用于筛选人λgt11表达文库。分离并测序了几个部分cDNA克隆。该蛋白的序列显示与NuMA相同，NuMA是一种236 kDa的核有丝分裂纺锤体装置蛋白。最近有两项独立研究对NuMA进行了表征，并且认为它是有丝分裂完成所需的蛋白质家族的一部分。在本报告中，使用cDNA克隆分析了NuMA基因的染色体定位和拷贝数。高分辨率原位杂交显示在人类11号染色体q13带的姐妹染色单体上有一对信号。对人类基因组DNA进行的严格Southern分析产生了简单的限制性图谱。这些结果共同表明，NuMA基因在人类11号染色体q13上以单拷贝形式存在。
