Takai S, Kozak C A, Kitamura K, Takeda A
Department of Genetics, International Medical Center of Japan, Tokyo, 162, Japan.
Genomics. 1996 Dec 15;38(3):429-31. doi: 10.1006/geno.1996.0648.
CD45-AP is a recently identified phosphorylated protein that specifically associates with the leukocyte-specific transmembrane glycoprotein CD45. The gene for CD45-AP, Ptprcap (protein tyrosine phosphatase, receptor type c polypeptide associated protein), was mapped in mouse by typing the progeny of two multilocus crosses using the mouse CD45-AP cDNA as a Southern hybridization probe. The CD45-AP gene mapped to the centromeric region of Chr 19 proximal to the genes Fth, Cd5, and Pcna-rs. The gene for the human CD45-AP homologue, PTPRCAP, was localized to chromosome band 11q13.1-q13.3 by fluorescence in situ hybridization using human genomic CD45-AP DNA as a hybridization probe. The genetic mapping of the Ptprcap/PTPRCAP genes extends the previously defined synteny conservation of various genes that have been assigned to these regions of the mouse and the human chromosomes.
CD45-AP是一种最近鉴定出的磷酸化蛋白，它特异性地与白细胞特异性跨膜糖蛋白CD45相关联。使用小鼠CD45-AP cDNA作为Southern杂交探针，通过对两个多位点杂交的后代进行分型，在小鼠中定位了CD45-AP的基因Ptprcap（蛋白酪氨酸磷酸酶，受体型c多肽相关蛋白）。CD45-AP基因定位于19号染色体的着丝粒区域，靠近Fth、Cd5和Pcna-rs基因。通过使用人类基因组CD45-AP DNA作为杂交探针进行荧光原位杂交，将人类CD45-AP同源物PTPRCAP的基因定位到染色体带11q13.1-q13.3。Ptprcap/PTPRCAP基因的遗传定位扩展了先前定义的已分配到小鼠和人类染色体这些区域的各种基因的同线性保守性。
