通过非放射性原位杂交和体细胞杂种将人类三肽基肽酶II基因（TPP2）定位于13q32 - q33。
Localization of the human tripeptidyl peptidase II gene (TPP2) to 13q32-q33 by nonradioactive in situ hybridization and somatic cell hybrids.
作者信息
Martinsson T, Vujic M, Tomkinson B
机构信息
Department of Clinical Genetics, East Hospital, University of Göteborg, Sweden.
出版信息
Genomics. 1993 Aug;17(2):493-5. doi: 10.1006/geno.1993.1353.
We have assigned the human tripeptidyl peptidase II (TPP2) gene to chromosome region 13q32-q33 using two different methods. First, a full-length TPP2 cDNA was used as a probe on Southern blots of DNA from a panel of human/rodent somatic cell hybrids. The TPP2 sequences were found to segregate with the human chromosome 13. Second, fluorescence in situ hybridization analysis was performed with the same probe. This analysis supported the chromosome 13 localization and further refined it to region 13q32-q33.
我们使用两种不同方法将人类三肽基肽酶II（TPP2）基因定位到染色体区域13q32 - q33。首先，使用全长TPP2 cDNA作为探针，对一组人/啮齿动物体细胞杂种的DNA进行Southern杂交。发现TPP2序列与人类13号染色体共分离。其次，用同一探针进行荧光原位杂交分析。该分析支持TPP2基因定位于13号染色体，并进一步将其精确定位到13q32 - q33区域。
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