Nevalainen T J, Haapanen T J
Department of Pathology, University of Turku, Finland.
Inflammation. 1993 Aug;17(4):453-64. doi: 10.1007/BF00916585.
The catalytic activity of phospholipase A2 (cat-PLA2) and the concentration of pancreatic (group I) phospholipase A2 (pan-PLA2) and synovial-type (group II) phospholipase A2 (syn-PLA2) were studied in 19 human tissues in order to find potential sources of circulating phospholipase A2. Five specimens of each tissue were collected at autopsies or from normal deliveries (placentas and amnionic membranes). The concentrations of pan-PLA2 and syn-PLA2 were measured by specific time-resolved fluoroimmunoassays. The cat-PLA2 was measured by radioactive dipalmitoyl phosphatidylcholine as substrate. The concentration of pan-PLA2 was negligible in all tissue homogenates except the pancreas. Immunoreactive syn-PLA2 was found in the homogenates of the digestive tract, cartilage, and prostatic and parotid glands. By immunohistochemistry, syn-PLA2 was localized in Paneth cell secretory granules, chondrocytes, cartilage matrix, and glandular cells of prostate.
为了寻找循环中磷脂酶A2的潜在来源，我们研究了19种人体组织中磷脂酶A2（催化型磷脂酶A2，cat-PLA2）的催化活性以及胰腺（I组）磷脂酶A2（pan-PLA2）和滑膜型（II组）磷脂酶A2（syn-PLA2）的浓度。在尸检时或正常分娩（胎盘和羊膜）过程中采集每种组织的5个样本。通过特异性时间分辨荧光免疫分析法测定pan-PLA2和syn-PLA2的浓度。以放射性二棕榈酰磷脂酰胆碱为底物测定cat-PLA2。除胰腺外，所有组织匀浆中pan-PLA2的浓度均可忽略不计。在消化道、软骨、前列腺和腮腺的匀浆中发现了免疫反应性syn-PLA2。通过免疫组织化学方法，syn-PLA2定位于潘氏细胞分泌颗粒、软骨细胞、软骨基质和前列腺腺细胞中。
