α-生育酚对人成纤维细胞培养物中抗氧化酶活性的影响。

Effects of alpha-tocopherol on antioxidant enzyme activity in human fibroblast cultures.

作者信息

Conti M, Couturier M, Lemonnier A, Lemonnier F

机构信息

Unité de Recherche génétique et mécanisme des maladies du foie de l'enfant INSERM U347, Hôpital de Bicêtre, France.

出版信息

Int J Vitam Nutr Res. 1993;63(2):71-6.

PMID:8407167

Abstract

The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (Cat) were determined in human fibroblast cultures at four concentrations of exogenous alpha-tocopherol: 0.2, 2.5, 10 and 50 micrograms/ml of culture medium, or without alpha-tocopherol. Relationships between alpha-tocopherol levels and the activities of SOD and GPx were identified. The cellular alpha-tocopherol level correlated with GPx activity (p < or = 0.01) and inversely correlated with SOD activity (p < or = 0.003), but only when alpha-tocopherol was added to the culture medium. The variations in the cellular GPx/SOD ratio depended on the level of cellular alpha-tocopherol (p < or = 0.001). Furthermore, there was a strong inverse correlation between SOD and GPx activity (p < or = 0.0001). Cat activity did not correlate either with cellular alpha-tocopherol concentration, or with SOD or GPx activity. These results underline the complex interplay between alpha-tocopherol and other antioxidant systems in human fibroblast cultures.

摘要

在人成纤维细胞培养物中，测定了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（Cat）在四种外源性α-生育酚浓度下的活性：培养基中分别含0.2、2.5、10和50微克/毫升α-生育酚，或不含α-生育酚。确定了α-生育酚水平与SOD和GPx活性之间的关系。细胞α-生育酚水平与GPx活性呈正相关（p≤0.01），与SOD活性呈负相关（p≤0.003），但仅在向培养基中添加α-生育酚时如此。细胞GPx/SOD比值的变化取决于细胞α-生育酚水平（p≤0.001）。此外，SOD和GPx活性之间存在强烈的负相关（p≤0.0001）。Cat活性既与细胞α-生育酚浓度无关，也与SOD或GPx活性无关。这些结果强调了α-生育酚与人类成纤维细胞培养物中其他抗氧化系统之间复杂的相互作用。