Michiels C, Toussaint O, Remacle J
Laboratoire de Biochimie Cellulaire, Facultés Universitaires ND de la Paix, Namur, Belgium.
J Cell Physiol. 1990 Aug;144(2):295-302. doi: 10.1002/jcp.1041440216.
The resistance of human pulmonary fibroblasts (WI-38) and human umbilical vein endothelial cells to oxygen toxicity (1 atm O2) was compared. Endothelial cells were more sensitive than fibroblasts. They contained also less antioxidant enzymes except for SOD: respectively 132%, 96%, 70%, 59%, and 21% of the SOD, GSH peroxidase, GSH reductase, catalase, and G6PD content of fibroblasts. However, they contained 1.81-fold more GSH than fibroblasts. Their lower content of antioxidant enzymes can explain their higher sensitivity to oxygen. The efficiency of natural antioxidant molecules and enzymes in the protection of cells incubated 3 days under 1 atm O2 was studied. alpha-tocopherol added in the culture medium led to a significant protection, contrary to the result for ascorbic acid. Microinjection of catalase, SOD, and GSH peroxidase directly into the cells was also tested: the protection was concentration dependent for both types of cells but SOD did not protect the endothelial cells. Lower activities of the other enzymes were needed to achieve protection of the endothelial cells, compared to fibroblasts. Since endothelial cells were also shown to display lower antioxidant enzyme activities, it can be hypothesized that their content is optimized for survival in physiological conditions.
比较了人肺成纤维细胞（WI-38）和人脐静脉内皮细胞对氧毒性（1个大气压氧气）的抗性。内皮细胞比成纤维细胞更敏感。除超氧化物歧化酶（SOD）外，它们含有的抗氧化酶也较少：内皮细胞的SOD、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、过氧化氢酶和葡萄糖-6-磷酸脱氢酶（G6PD）的含量分别是成纤维细胞的132%、96%、70%、59%和21%。然而，它们的谷胱甘肽（GSH）含量比成纤维细胞多1.81倍。它们较低的抗氧化酶含量可以解释其对氧更高的敏感性。研究了天然抗氧化分子和酶在保护细胞于1个大气压氧气下培养3天中的效率。向培养基中添加α-生育酚可产生显著的保护作用，这与抗坏血酸的结果相反。还测试了将过氧化氢酶、SOD和谷胱甘肽过氧化物酶直接显微注射到细胞中的情况：对于两种类型的细胞，保护作用均呈浓度依赖性，但SOD对内皮细胞没有保护作用。与成纤维细胞相比，保护内皮细胞需要较低活性的其他酶。由于内皮细胞也显示出较低的抗氧化酶活性，因此可以推测其含量针对在生理条件下的存活进行了优化。
