Luo W, al-Abdulaly A B, Yoon K, Simpson K L
Department of Food Science and Nutrition, University of Rhode Island, Kingston 02881.
Int J Vitam Nutr Res. 1993;63(2):82-6.
An aqueous, reverse phase, open column procedure (RP C18) was developed for the rapid determination of retinol in serum. The samples were extracted with hexane and separated on the column with methanol and water as the mobile phase. Two methods were developed depending on the size of the sample. For 2 ml of serum a spectrophotometer was used to quantify the sample and for 0.1 ml of serum a fluorometer was used. Phytofluene was retained on the top of the column and the column was reusable. The agreement between HPLC and the RP C18 open column was good. The lower limit of detection for micromethod was about 1.25 micrograms of retinol per dl of serum and the recovery of added retinol was quantitative.
开发了一种用于快速测定血清中视黄醇的反相开放柱水相程序（RP C18）。样品用己烷萃取，以甲醇和水为流动相在柱上分离。根据样品量开发了两种方法。对于2 ml血清，使用分光光度计对样品进行定量；对于0.1 ml血清，使用荧光计。六氢番茄红素保留在柱顶，该柱可重复使用。高效液相色谱法（HPLC）与RP C18开放柱法之间的一致性良好。微量法的检测下限约为每分升血清1.25微克视黄醇，添加视黄醇的回收率为定量。
