Ortiz A, Teruel J A
Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad de Murcia, Spain.
Biochem Int. 1991 Oct;25(3):429-35.
A simple isocratic high performance liquid chromatograph (HPLC) system is described to perform a rapid separation, identification and quantitative determination of vitamin E (alpha-tocopherol) in biological membranes. It makes use of a reverse phase C18 column with pure methanol as the mobile phase, and an ultraviolet detector which enables its quantification in the nanogram scale. This procedure was applied to lipid extracts from whole muscle homogenate and from a preparation of sarcoplasmic reticulum vesicles from skeletal muscle, where the vitamin E contents was determined.
本文描述了一种简单的等度高效液相色谱（HPLC）系统，用于在生物膜中快速分离、鉴定和定量测定维生素E（α-生育酚）。该系统采用反相C18柱，以纯甲醇作为流动相，并配备紫外检测器，可实现纳克级别的定量分析。此方法应用于全肌肉匀浆的脂质提取物以及骨骼肌肌质网囊泡制剂，测定其中维生素E的含量。
