Sano Y, Kobayashi M, Kageyama M
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
J Bacteriol. 1993 Oct;175(19):6179-85. doi: 10.1128/jb.175.19.6179-6185.1993.
Functional domain structures of pyocins AP41, S1, and S2 were assigned by examining the functions of chimeric pyocins and deletion derivatives. Pyocins AP41, S1, and S2 are essentially composed of three domains, the receptor-binding domain, the translocation domain, and the DNase domain, in that order from the N terminus to the C terminus. The alignment of these domains is distinct from that in E2-group colicins with functions similar to those of these pyocins. Pyocins AP41 and S2 have a fourth domain between the receptor-binding and the translocation domains, which is dispensable for their killing functions.
通过研究嵌合型绿脓菌素和缺失衍生物的功能，确定了绿脓菌素AP41、S1和S2的功能结构域。绿脓菌素AP41、S1和S2基本上由三个结构域组成，即受体结合结构域、转运结构域和DNase结构域，从N端到C端依次排列。这些结构域的排列与功能类似于这些绿脓菌素的E2组大肠杆菌素不同。绿脓菌素AP41和S2在受体结合结构域和转运结构域之间有第四个结构域，该结构域对它们的杀伤功能来说是可有可无的。
