Krauwinkel W J, Volkers-Kamermans N J, van Zijtveld J
Department of Biopharmaceutical Research, Brocades Pharma R&D, Leiderdorp, Netherlands.
J Chromatogr. 1993 Aug 11;617(2):334-8. doi: 10.1016/0378-4347(93)80509-3.
A high-performance liquid chromatographic method using solid phase extraction has been developed for the determination of amoxicillin in human plasma. After concentration of amoxicillin on a C8 cartridge, the cartridge was eluted on-line to a reversed-phase column packed with 5 microns Chromspher C18. The mobile phase was methanol-0.08 M phosphate buffer (pH 7.6) (20:80), which contained 0.01 M tetrabutylammonium dihydrogenphosphate. The column effluent was monitored by UV spectrophotometry at 234 nm. The method has proved to be reliable and is used in bioavailability studies for the development of new amoxicillin formulations.
已开发出一种使用固相萃取的高效液相色谱法来测定人血浆中的阿莫西林。在C8柱上对阿莫西林进行浓缩后，将该柱在线洗脱至填充有5微米Chromspher C18的反相柱上。流动相为甲醇-0.08 M磷酸盐缓冲液（pH 7.6）（20:80），其中含有0.01 M磷酸二氢四丁铵。通过紫外分光光度法在234 nm处监测柱流出物。该方法已被证明可靠，并用于新阿莫西林制剂开发的生物利用度研究。
