Yuan Z, Russlie H Q, Canafax D M
Department of Pharmacy Practice, College of Pharmacy and School of Medicine, University of Minnesota, Minneapolis, USA.
J Chromatogr B Biomed Appl. 1995 Dec 1;674(1):93-9. doi: 10.1016/0378-4347(95)00302-1.
We developed a sensitive assay to measure amoxicillin in human plasma and middle ear fluid (MEF) using solid-phase extraction and reversed-phase HPLC. Amoxicillin and cefadroxil, the internal standard, were extracted from 50-200 microliters of sample with Bond Elut C18 cartridges. The extract was analyzed on a 15 cm x 2 mm, 5 micron Keystone MOS Hypersil-1 (C8) column with UV detection at 210 nm. The mobile phase was 6% acetonitrile in 5 mM phosphate buffer (pH = 6.5) and 5 mM tetrabutylammonium. The average absolute recovery of amoxicillin and cefadroxil were 91.2 +/- 16.6% and 91.0 +/- 6.8%, respectively. The limit of quantitation was 0.125 microgram/ml with 200 microliters sample size. The linear range was from 0.125 to 35.0 micrograms/ml with correlation coefficients greater than 0.999. These analytic conditions produced a highly sensitive amoxicillin assay in human body fluids without derivatization.
我们开发了一种灵敏的检测方法，采用固相萃取和反相高效液相色谱法测定人血浆和中耳液（MEF）中的阿莫西林。阿莫西林和内标头孢羟氨苄用Bond Elut C18柱从50 - 200微升样品中萃取。萃取物在一根15 cm×2 mm、5微米的Keystone MOS Hypersil - 1（C8）柱上进行分析，在210 nm处进行紫外检测。流动相为含6%乙腈的5 mM磷酸盐缓冲液（pH = 6.5）和5 mM四丁基铵。阿莫西林和头孢羟氨苄的平均绝对回收率分别为91.2±16.6%和91.0±6.8%。当进样量为200微升时，定量限为0.125微克/毫升。线性范围为0.125至35.0微克/毫升，相关系数大于0.999。这些分析条件无需衍生化即可在人体体液中产生高灵敏度的阿莫西林检测方法。
