Matsuura A, Nagayama T, Kitagawa T
Shionogi Research Laboratories, Shionogi & Co., Ltd., Osaka, Japan.
J Chromatogr. 1993 Aug 11;617(2):339-43. doi: 10.1016/0378-4347(93)80510-b.
An automated high-performance liquid chromatographic method for the determination of the diuretic drug furosemide has been established. Dog plasma was injected directly into a two-column system with a BSA-ODS (ODS column coated with bovine serum albumin) precolumn and a C18 analytical column for the separation of furosemide. The two columns were automatically switched. Furosemide remained trapped on the precolumn while proteins were eluted to waste. After column switching, furosemide was washed onto the analytical column and analysed without interference. The greatest advantage of the method is its easy performance without manual sample preparation; it requires no extraction or deproteinization. The method allows determination of 0.1-10 micrograms/ml of furosemide with accuracy and precision comparable with previously reported values. The coefficients of variation obtained from replicate measurements of 1 microgram/ml and 5 micrograms/ml samples were 1.65% and 2.40%, respectively. This method was used to measure the plasma levels of furosemide in beagle dogs to whom the drugs was administered, as a reference, in a toxicological study.
已建立一种用于测定利尿药呋塞米的高效液相色谱自动化方法。将犬血浆直接注入一个双柱系统，该系统配有一个牛血清白蛋白涂覆的十八烷基硅烷键合硅胶（BSA-ODS，即涂有牛血清白蛋白的ODS柱）预柱和一个C18分析柱，用于分离呋塞米。两根柱子自动切换。呋塞米保留在预柱上，而蛋白质被洗脱至废液。柱切换后，呋塞米被冲洗到分析柱上并进行分析，无干扰。该方法的最大优点是无需手动样品制备即可轻松操作；无需萃取或去蛋白。该方法能够测定浓度为0.1 - 10微克/毫升的呋塞米，其准确度和精密度与先前报道的值相当。对1微克/毫升和5微克/毫升样品进行重复测量得到的变异系数分别为1.65%和2.40%。在一项毒理学研究中，该方法被用于测量给药后的比格犬血浆中呋塞米的水平作为参考。
