Rady P L, Chin R, Arany I, Hughes T K, Tyring S K
Department of Microbiology, University of Texas, Medical Branch, Galveston 77550.
J Virol Methods. 1993 Aug;43(3):335-50. doi: 10.1016/0166-0934(93)90151-g.
Consensus primers specific for the L1 and E1 regions were used to amplify HPV DNA fragments. These fragments were then directly sequenced using the consensus primers as the sequencing primers. The linking of PCR amplification, direct sequencing, and computer data bank analysis provides a new approach in the detection of different HPVs and has several advantages over existing methodology. These advantages include increased precision in the rapid characterization of known HPVs, detection of mutations, and identification of new HPV types.
使用针对L1和E1区域的共有引物来扩增人乳头瘤病毒（HPV）DNA片段。然后使用这些共有引物作为测序引物对这些片段进行直接测序。聚合酶链反应（PCR）扩增、直接测序和计算机数据库分析相结合，为检测不同的HPV提供了一种新方法，与现有方法相比具有多个优势。这些优势包括在快速鉴定已知HPV、检测突变以及识别新的HPV类型方面提高了准确性。
