一种生物发光分枝杆菌的构建及其在抗分枝杆菌药物测定中的应用。
Construction of a bioluminescent mycobacterium and its use for assay of antimycobacterial agents.
作者信息
Andrew P W, Roberts I S
机构信息
Department of Microbiology, University of Leicester, United Kingdom.
出版信息
J Clin Microbiol. 1993 Sep;31(9):2251-4. doi: 10.1128/jcm.31.9.2251-2254.1993.
Abstract
To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of measuring antimycobacterial activity, a bioluminescent form of Mycobacterium smegmatis was made by transformation with a Mycobacterium-Escherichia coli shuttle vector containing the luxAB genes from Vibrio harveyi. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.
摘要
为了作为一个模型系统证明分枝杆菌能够表达异源荧光素酶基因，且生物发光可作为一种快速测定抗分枝杆菌活性的方法，通过用含有来自哈维弧菌的luxAB基因的分枝杆菌-大肠杆菌穿梭载体转化，构建了耻垢分枝杆菌的生物发光形式。利用生物发光的耻垢分枝杆菌可实时测定抗生素和杀菌剂的抗分枝杆菌效果。
相似文献
1
Construction of a bioluminescent mycobacterium and its use for assay of antimycobacterial agents.一种生物发光分枝杆菌的构建及其在抗分枝杆菌药物测定中的应用。
J Clin Microbiol. 1993 Sep;31(9):2251-4. doi: 10.1128/jcm.31.9.2251-2254.1993.
2
Bioluminescent Mycobacterium aurum expressing firefly luciferase for rapid and high throughput screening of antimycobacterial drugs in vitro and in infected macrophages.表达萤火虫荧光素酶的生物发光金色分枝杆菌用于体外和感染巨噬细胞中抗分枝杆菌药物的快速高通量筛选。
Biochem Biophys Res Commun. 2000 Dec 20;279(2):457-61. doi: 10.1006/bbrc.2000.3957.
3
Bioluminescence screening in vitro (Bio-Siv) assays for high-volume antimycobacterial drug discovery.用于高通量抗分枝杆菌药物发现的体外生物发光筛选（Bio-Siv）测定法。
Antimicrob Agents Chemother. 1996 Jun;40(6):1536-41. doi: 10.1128/AAC.40.6.1536.
4
A stable Escherichia coli-Mycobacterium smegmatis plasmid shuttle vector containing the mycobacteriophage D29 origin.一种含有分枝杆菌噬菌体D29复制起点的稳定的大肠杆菌-耻垢分枝杆菌质粒穿梭载体。
Plasmid. 1992 Nov;28(3):267-71. doi: 10.1016/0147-619x(92)90059-j.
5
Reporter gene technology to assess activity of antimycobacterial agents in macrophages.利用报告基因技术评估巨噬细胞中抗分枝杆菌药物的活性。
Antimicrob Agents Chemother. 1996 Jun;40(6):1542-4. doi: 10.1128/AAC.40.6.1542.
6
The use of luciferase-reporter phage for antibiotic-susceptibility testing of mycobacteria.荧光素酶报告噬菌体在分枝杆菌抗生素敏感性检测中的应用。
Methods Mol Biol. 1998;101:431-55. doi: 10.1385/0-89603-471-2:431.
7
Acetone leaf extracts of some South African trees with high activity against Escherichia coli also have good antimycobacterial activity and selectivity index.一些对大肠杆菌具有高活性的南非树木的丙酮叶提取物也具有良好的抗分枝杆菌活性和选择性指数。
BMC Complement Altern Med. 2017 Jun 19;17(1):327. doi: 10.1186/s12906-017-1831-z.
8
The application of luciferase as a reporter of environmental regulation of gene expression in mycobacteria.
Lett Appl Microbiol. 1994 Nov;19(5):336-40. doi: 10.1111/j.1472-765x.1994.tb00469.x.
9
The beta subunit polypeptide of Vibrio harveyi luciferase determines light emission at 42 degrees C.哈维氏弧菌荧光素酶的β亚基多肽决定了在42摄氏度时的发光情况。
Mol Gen Genet. 1991 Dec;230(3):385-93. doi: 10.1007/BF00280295.
10
Highly bioluminescent Bacillus subtilis obtained through high-level expression of a luxAB fusion gene.
Mol Gen Genet. 1991 Nov;230(1-2):251-6. doi: 10.1007/BF00290675.
引用本文的文献
1
Rapid Tuberculosis Diagnosis Using Reporter Enzyme Fluorescence.利用报告酶荧光快速诊断结核病。
J Clin Microbiol. 2019 Nov 22;57(12). doi: 10.1128/JCM.01462-19. Print 2019 Dec.
2
In-vivo monitoring of infectious diseases in living animals using bioluminescence imaging.活体动物体内传染病的生物发光成像监测。
Virulence. 2018 Jan 1;9(1):28-63. doi: 10.1080/21505594.2017.1371897. Epub 2017 Dec 8.
3
A Fluorescent Probe for Detecting and Identifying Genes Critical for Cell Entry.一种用于检测和鉴定细胞进入关键基因的荧光探针。
Front Microbiol. 2016 Dec 20;7:2021. doi: 10.3389/fmicb.2016.02021. eCollection 2016.
4
Intravital excitation increases detection sensitivity for pulmonary tuberculosis by whole-body imaging with β-lactamase reporter enzyme fluorescence.活体激发通过β-内酰胺酶报告酶荧光全身成像提高肺结核检测灵敏度。
J Biophotonics. 2017 Jun;10(6-7):821-829. doi: 10.1002/jbio.201600132. Epub 2016 Oct 18.
5
Bioluminescence for assessing drug potency against nonreplicating Mycobacterium tuberculosis.用于评估抗非复制型结核分枝杆菌药物效力的生物发光法。
Antimicrob Agents Chemother. 2015 Jul;59(7):4012-9. doi: 10.1128/AAC.00528-15. Epub 2015 Apr 20.
6
Real-time bioluminescence imaging of mixed mycobacterial infections.混合分枝杆菌感染的实时生物发光成像
PLoS One. 2014 Sep 29;9(9):e108341. doi: 10.1371/journal.pone.0108341. eCollection 2014.
7
Simple and rapid method to determine antimycobacterial potency of compounds by using autoluminescent Mycobacterium tuberculosis.利用自发光结核分枝杆菌测定化合物抗分枝杆菌效力的简单快速方法。
Antimicrob Agents Chemother. 2014 Oct;58(10):5801-8. doi: 10.1128/AAC.03205-14. Epub 2014 Jul 21.
8
Comparison of quantitative techniques including Xpert MTB/RIF to evaluate mycobacterial burden.比较包括 Xpert MTB/RIF 在内的定量技术评估分枝杆菌负荷。
PLoS One. 2011;6(12):e28815. doi: 10.1371/journal.pone.0028815. Epub 2011 Dec 22.
9
Reporter enzyme fluorescence (REF) imaging and quantification of tuberculosis in live animals.实时动物体内结核Reporter 酶荧光（REF）成像和定量。
Virulence. 2010 Nov-Dec;1(6):558-62. doi: 10.4161/viru.1.6.13901. Epub 2010 Nov 1.
10
Imaging tuberculosis with endogenous beta-lactamase reporter enzyme fluorescence in live mice.利用内源性β-内酰胺酶报告酶荧光在活鼠中成像结核分枝杆菌。
Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12239-44. doi: 10.1073/pnas.1000643107. Epub 2010 Jun 21.
本文引用的文献
1
Disinfection of the flexible fibreoptic bronchoscope against Mycobacterium tuberculosis and M gordonae.软性纤维支气管镜针对结核分枝杆菌和戈登分枝杆菌的消毒
Thorax. 1984 Oct;39(10):785-8. doi: 10.1136/thx.39.10.785.
2
3
Kinetics of the tuberculocidal response by alkaline glutaraldehyde in solution and on an inert surface.
J Appl Bacteriol. 1986 Jul;61(1):87-93. doi: 10.1111/j.1365-2672.1986.tb03762.x.
4
In vitro observations on the suitability of new rifamycins for the intermittent chemotherapy of tuberculosis.关于新型利福霉素用于结核病间歇化疗适用性的体外观察
Tubercle. 1987 Sep;68(3):183-93. doi: 10.1016/0041-3879(87)90054-7.
5
Tuberculosis and human immunodeficiency virus infection.
J Infect Dis. 1989 Jan;159(1):96-100. doi: 10.1093/infdis/159.1.96.
6
HIV infection and tuberculosis.艾滋病毒感染与结核病。
BMJ. 1990 Jan 13;300(6717):63-5. doi: 10.1136/bmj.300.6717.63.
7
Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis.耻垢分枝杆菌高效质粒转化突变体的分离与鉴定
Mol Microbiol. 1990 Nov;4(11):1911-9. doi: 10.1111/j.1365-2958.1990.tb02040.x.
8
PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products.基于聚合酶链反应（PCR）对哈维氏弧菌lux操纵子和大肠杆菌trp操纵子进行基因工程改造，可产生具有生化功能的天然和融合基因产物。
Mol Gen Genet. 1991 Apr;226(1-2):41-8. doi: 10.1007/BF00273585.
9
New use of BCG for recombinant vaccines.卡介苗在重组疫苗中的新用途。
Nature. 1991 Jun 6;351(6326):456-60. doi: 10.1038/351456a0.
10
The global tuberculosis situation and the new control strategy of the World Health Organization.全球结核病状况及世界卫生组织的新控制策略
Tubercle. 1991 Mar;72(1):1-6. doi: 10.1016/0041-3879(91)90017-m.
Zotero 插件