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一种生物发光分枝杆菌的构建及其在抗分枝杆菌药物测定中的应用。

Construction of a bioluminescent mycobacterium and its use for assay of antimycobacterial agents.

作者信息

Andrew P W, Roberts I S

机构信息

Department of Microbiology, University of Leicester, United Kingdom.

出版信息

J Clin Microbiol. 1993 Sep;31(9):2251-4. doi: 10.1128/jcm.31.9.2251-2254.1993.

DOI:10.1128/jcm.31.9.2251-2254.1993
PMID:8408541
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265743/
Abstract

To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of measuring antimycobacterial activity, a bioluminescent form of Mycobacterium smegmatis was made by transformation with a Mycobacterium-Escherichia coli shuttle vector containing the luxAB genes from Vibrio harveyi. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.

摘要

为了作为一个模型系统证明分枝杆菌能够表达异源荧光素酶基因，且生物发光可作为一种快速测定抗分枝杆菌活性的方法，通过用含有来自哈维弧菌的luxAB基因的分枝杆菌-大肠杆菌穿梭载体转化，构建了耻垢分枝杆菌的生物发光形式。利用生物发光的耻垢分枝杆菌可实时测定抗生素和杀菌剂的抗分枝杆菌效果。