Albert M J, Ansaruzzaman M, Bhuiyan N A
International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh.
J Diarrhoeal Dis Res. 1993 Jun;11(2):101-4.
Current evidence suggests that enteropathogenic Escherichia coli (EPEC) of traditional serotypes possess a three-stage pathogenesis: localised adherence (LA), to, attachment-effacement (AE) of, and penetration of, enterocytes, all of which can be reproduced in tissue culture models in vitro. Three E. coli isolates of non-traditional serotypes (02:H2, 02:H25 and 015:H2) isolated from children with diarrhoea were previously shown to be positive for LA and AE activities in laboratory models. In the present study, they were, in addition, shown to be positive for invasion of a HEp-2 cell monolayer. These findings further establish the pathogenicity of non-traditional serotypes of E. coli and their role in the causation of diarrhoea.
目前的证据表明，传统血清型的肠致病性大肠杆菌（EPEC）具有三个致病阶段：局部黏附（LA）、对肠上皮细胞的黏附-抹消（AE）以及穿透，所有这些在体外组织培养模型中均可重现。先前从腹泻儿童中分离出的三株非传统血清型大肠杆菌（02:H2、02:H25和015:H2）在实验室模型中被证明具有LA和AE活性。在本研究中，它们还被证明对HEp-2细胞单层具有侵袭性。这些发现进一步证实了非传统血清型大肠杆菌的致病性及其在腹泻病因中的作用。
