Takizawa T, Robinson J M
Department of Cell Biology, Neurobiology, and Anatomy, Ohio State University, Columbus.
J Histochem Cytochem. 1993 Nov;41(11):1635-9. doi: 10.1177/41.11.8409371.
We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultrathin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.
我们将免疫细胞化学和酶细胞化学相结合，以便在同一超薄冰冻切片上定位两种不同的蛋白质。在该方法中，免疫细胞化学定位通过胶体金探针可视化，酶细胞化学检测则以铈作为捕获剂来实现。先进行免疫细胞化学，这样酶细胞化学过程的任何潜在不利影响都不会改变冰冻切片的抗体结合特性。
