Rowden G, Dean S
Pathology Department, Dalhousie University, Halifax, Nova Scotia, Canada.
J Electron Microsc Tech. 1991 Jun;18(2):121-5. doi: 10.1002/jemt.1060180205.
Immunoelectron microscopic localization of surface antigens on lymphocytes is possible using alkaline phosphatase combined with cerium-based cytochemical methods. Distinctive electron-dense deposits are easily identified at sites of antibody binding. Mouse splenocytes showing surface immunoglobulin localization and human peripheral blood lymphocytes stained for the MHC-Class II antigen HLA-DR illustrate the results. Double staining for murine Ia antigen by the alkaline phosphatase procedure, combined with immunogold labeling of antigens identified on dendritic cells, i.e., NLDC-145, demonstrates the utility of the cerium cytochemical method.
利用碱性磷酸酶结合基于铈的细胞化学方法，可以对淋巴细胞表面抗原进行免疫电子显微镜定位。在抗体结合部位很容易识别出独特的电子致密沉积物。显示表面免疫球蛋白定位的小鼠脾细胞以及针对MHC-II类抗原HLA-DR染色的人外周血淋巴细胞说明了该结果。通过碱性磷酸酶法对鼠Ia抗原进行双重染色，并结合对树突状细胞上鉴定出的抗原（即NLDC-145）进行免疫金标记，证明了铈细胞化学方法的实用性。
