Kushimoto T, Yokozumi K, Ogihara Y, Yatani R, Imahara T
Department of Orthopaedic Surgery, Mie University School of Medicine, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1993 Sep;67(9):836-46.
To clarify the histogenesis of malignant fibrous histiocytoma (MFH), ovoid and spindle cells, the major components of this tumor, were biologically and morphologically studied using the following methods: (1) flow cytometric nuclear DNA analysis, (2) argyrophil staining for nucleolar organizer regions (Ag-NORs), (3) separation of MFH cells into the two types described above using a flow cytometric cell sorting system, (4) electron microscopic morphological observation, and (5) immunohistochemical examination. Specimens were obtained from 24 cases of soft-tissue MFH and 7 cases of MFH in the bone, which were treated in our institute. In ovoid cells, aneuploid pattern was shown by nuclear DNA analysis and high proliferation activity was found on Ag-NORs staining. Ultrastructural analysis showed that the ovoid cells had malignant characteristics and were presumedly histiocytic in origin. Immunohistochemical examination also, supported histiocytic origin. However, spindle cells were not identified as malignant tumor cells by the various methods described above. Although the histogenesis of MFH remains to be elucidated, these findings provide interesting information.
为阐明恶性纤维组织细胞瘤（MFH）的组织发生，对该肿瘤的主要成分卵圆形细胞和梭形细胞进行了生物学和形态学研究，采用了以下方法：（1）流式细胞术核DNA分析；（2）核仁组成区嗜银染色（Ag-NORs）；（3）使用流式细胞术细胞分选系统将MFH细胞分为上述两种类型；（4）电子显微镜形态学观察；（5）免疫组织化学检查。标本取自本院治疗的24例软组织MFH和7例骨MFH。在卵圆形细胞中，核DNA分析显示非整倍体模式，Ag-NORs染色发现高增殖活性。超微结构分析表明，卵圆形细胞具有恶性特征，推测起源于组织细胞。免疫组织化学检查也支持其组织细胞起源。然而，上述各种方法均未将梭形细胞鉴定为恶性肿瘤细胞。尽管MFH的组织发生仍有待阐明，但这些发现提供了有趣的信息。
