Tang L, Lucas A H, Eaton J W
Biomedical Engineering Graduate Program, University of Minnesota.
J Lab Clin Med. 1993 Sep;122(3):292-300.
In many cases, evidently inert and nontoxic biomaterials may trigger procoagulant and inflammatory responses. Because most polymeric biomaterials accumulate a surface layer of protein immediately after implantation, these adverse reactions may stem from secondary interactions between the host and this surface layer of adsorbed proteins. Using polyester terephthalate (the polymer from which both Dacron and Mylar are produced) as a model, we have explored the hypothesis that surface-adsorbed immunoglobulin might mediate subsequent inflammatory responses. We find, as have others, that immunoglobulin G (IgG) does spontaneously adsorb to polymer surfaces, both in vitro and in vivo. Furthermore, polymer implants precoated with IgG do activate human polymorphonuclear neutrophils in vitro and also attract substantial numbers of phagocytes (especially polymorphonuclear neutrophils and macrophages) when implanted in mice. However, when implants are placed in mice having a form of severe combined immunodeficiency (and, consequently, almost undetectable levels of serum IgG), a near-normal influx of phagocytic cells ensues. Thus, spontaneously-adsorbed surface IgG does not appear to be a necessary precedent to inflammatory responses directed against implanted biomaterials.
在许多情况下，明显惰性且无毒的生物材料可能引发促凝血和炎症反应。由于大多数聚合生物材料在植入后会立即在表面堆积一层蛋白质，这些不良反应可能源于宿主与这层吸附蛋白质表面层之间的二次相互作用。以聚对苯二甲酸乙二酯（生产涤纶和聚酯薄膜的聚合物）为模型，我们探究了表面吸附的免疫球蛋白可能介导后续炎症反应的假说。我们和其他人一样发现，免疫球蛋白G（IgG）确实会在体外和体内自发吸附到聚合物表面。此外，预先用IgG包被的聚合物植入物在体外确实会激活人类多形核中性粒细胞，并且在植入小鼠体内时也会吸引大量吞噬细胞（尤其是多形核中性粒细胞和巨噬细胞）。然而，当将植入物置于患有某种严重联合免疫缺陷（因此血清IgG水平几乎检测不到）的小鼠体内时，仍会出现近乎正常的吞噬细胞流入。因此，自发吸附的表面IgG似乎并非针对植入生物材料的炎症反应的必要先决条件。
