Tiran A, Tiran B, Hojas S, Kostner G M, Wilders-Truschnig M M
Medizinische Universitätsklinik, Karl-Franzens, Universität, Graz, Austria.
J Clin Lab Anal. 1993;7(5):256-62. doi: 10.1002/jcla.1860070504.
A new fully automated nephelometric immunoassay for lipoprotein(a) quantification in human serum was evaluated using the Behring Nephelometer Analyzer. The assay exhibited a good linearity in the concentration range of 110-1,770 mg/l; at higher concentrations, samples were automatically diluted by a factor of 4. The method is simple, robust, and shows an excellent stability of the calibration curve over several weeks. Intra-assay and day-to-day coefficients of variation were 2% and 4.5%, respectively. The method correlated well with electroimmunodiffusion (r = 0.977; n = 123; P = 0.0001). Unspecific turbidity as expressed by an elevated blank value occurred in 3% of all freshly measured samples (n = 392). Storage of the samples for 1 week at 4 degrees C had no significant influence on the results. Frozen sera, on the other hand, cannot be assayed by this method. We believe that this assay is well suited for use in clinical routine work.
使用贝林散射比浊分析仪对一种用于定量检测人血清中脂蛋白(a)的新型全自动散射比浊免疫分析方法进行了评估。该分析方法在110 - 1770 mg/l的浓度范围内呈现出良好的线性；在更高浓度下，样品会自动稀释4倍。该方法简便、稳健，并且校准曲线在数周内具有出色的稳定性。批内和日间变异系数分别为2%和4.5%。该方法与电免疫扩散法相关性良好（r = 0.977；n = 123；P = 0.0001）。在所有新检测的样品（n = 392）中，有3%出现了由空白值升高所表示的非特异性浊度。样品在4℃下储存1周对结果无显著影响。另一方面，冷冻血清不能用该方法进行检测。我们认为这种分析方法非常适合用于临床常规工作。
