Jandreski M A, Garbincius J
Department of Pathology, Loyola University Medical Center, Maywood, Illinois 60153.
J Clin Lab Anal. 1993;7(5):263-8. doi: 10.1002/jcla.1860070505.
Occasionally, requests are made by our physicians for the measurement of gentamicin, tobramycin, or vancomycin in cerebrospinal fluid (CSF) specimens during the course of treating patients for bacterial meningitis. We evaluated CSF as a specimen type for the measurement of amikacin, gentamicin, tobramycin, and vancomycin on the Abbott TDx analyzer. Coefficients of variation for CSF spiked with these antimicrobial agents ranged from 0.8% to 6.5% for intra-assay values and from 2.1% to 2.3% for inter-assay values. Serum and CSF specimens were spiked at various levels with equal amounts of the antibiotics. Correlation coefficients for serum vs. CSF for these agents were 0.999. Recoveries ranged from 86% to 134%. Sensitivity for these assays is about fourfold better for CSF than for serum. CSF appears to be an acceptable specimen type for the measurement of these antibiotics using the Abbott TDx analyzer.
在治疗细菌性脑膜炎患者的过程中，我们的医生偶尔会要求检测脑脊液（CSF）样本中的庆大霉素、妥布霉素或万古霉素。我们评估了脑脊液作为样本类型在雅培TDx分析仪上检测阿米卡星、庆大霉素、妥布霉素和万古霉素的情况。添加这些抗菌药物的脑脊液样本批内变异系数为0.8%至6.5%，批间变异系数为2.1%至2.3%。血清和脑脊液样本用等量的抗生素进行不同水平的加样。这些药物血清与脑脊液的相关系数为0.999。回收率在86%至134%之间。这些检测对脑脊液的灵敏度比对血清的灵敏度约高四倍。脑脊液似乎是使用雅培TDx分析仪检测这些抗生素的可接受样本类型。
