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聚合酶链反应扩增、克隆及对变异大肠杆菌II型志贺样毒素操纵子的测序

Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.

作者信息

Paton A W, Paton J C, Manning P A

机构信息

Department of Microbiology, Adelaide Children's Hospital, South Australia.

出版信息

Microb Pathog. 1993 Jul;15(1):77-82. doi: 10.1006/mpat.1993.1058.

DOI:10.1006/mpat.1993.1058
PMID:8412629
Abstract

The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.

摘要

采用聚合酶链反应（PCR）从大肠杆菌血清型OX3:H21和O111:H-中扩增出约1.5 kb包含完整II型志贺样毒素操纵子的DNA片段。将这些片段克隆，DNA序列分析显示与已发表的SLT-II序列相比存在更多变异。

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