聚合酶链反应扩增、克隆及对变异大肠杆菌II型志贺样毒素操纵子的测序
Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons.
作者信息
Paton A W, Paton J C, Manning P A
机构信息
Department of Microbiology, Adelaide Children's Hospital, South Australia.
出版信息
Microb Pathog. 1993 Jul;15(1):77-82. doi: 10.1006/mpat.1993.1058.
PMID:8412629
Abstract
The polymerase chain reaction (PCR) was used to amplify approximately 1.5 kb segments of DNA containing complete Shiga-like toxin type II operons from Escherichia coli serotypes OX3:H21 and O111:H-. These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences.
摘要
采用聚合酶链反应（PCR）从大肠杆菌血清型OX3:H21和O111:H-中扩增出约1.5 kb包含完整II型志贺样毒素操纵子的DNA片段。将这些片段克隆，DNA序列分析显示与已发表的SLT-II序列相比存在更多变异。
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