一种新发现的由牛产志贺毒素大肠杆菌产生的志贺毒素变体，VT2g。
A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli.
作者信息
Leung P H M, Peiris J S M, Ng W W S, Robins-Browne R M, Bettelheim K A, Yam W C
机构信息
Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, People's Republic of China.
出版信息
Appl Environ Microbiol. 2003 Dec;69(12):7549-53. doi: 10.1128/AEM.69.12.7549-7553.2003.
Abstract
A new verotoxin (VT) variant, designated vt2g, was identified from a bovine strain of verocytotoxigenic Escherichia coli (VTEC) serotype O2:H25. When vt2g was aligned with published sequences of vt2 and vt variants, it exhibited the highest DNA sequence homology with vt2 and vt2c. However, vt2g was not detected by vt2-specific primers and probes, although it was partially neutralized by an antiserum to the VT2A subunit. VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-.
摘要
从一株血清型为O2:H25的产志贺毒素大肠杆菌（VTEC）牛源菌株中鉴定出一种新的志贺毒素（VT）变体，命名为vt2g。当将vt2g与已发表的vt2和vt变体序列进行比对时，它与vt2和vt2c表现出最高的DNA序列同源性。然而，尽管vt2g能被针对VT2A亚基的抗血清部分中和，但用vt2特异性引物和探针却检测不到它。VT2g对Vero细胞和HeLa细胞具有细胞毒性，且不被小鼠肠黏液激活。在409株牛VTEC菌株中有3株（0.7%）检测到vt2g基因，包括血清型O2:H25、O2:H45和Ont:H- 。
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