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从不同食品中分离出的植物乳杆菌菌株所产生的细菌素的检测。

Detection of bacteriocins produced by Lactobacillus plantarum strains isolated from different foods.

作者信息

Atrih A, Rekhif N, Michel M, Lefebvre G

机构信息

Laboratoire de Microbiologie Alimentaire, Industries Alimentaires-Institut National Polytechnique de Lorraine, Vandoeuvre-lès-Nancy, France.

出版信息

Microbios. 1993;75(303):117-23.

PMID:8412846
Abstract

Bacterial strains (106 in toto) isolated from different foods and identified as Lactobacillus plantarum were screened for antagonistic activities against other bacteria under conditions which eliminated the effects of organic acids and hydrogen peroxide. Five isolates were shown to be bacteriocin producers, and the bacteriocins, on the basis of their host range inhibition and cross inhibition were all different. The bacteriocins were preliminarily characterized by temperature stability, sensitivity to proteolytic, lipolytic and glycolytic enzymes and precipitation with ammonium sulphate.

摘要

从不同食品中分离出并鉴定为植物乳杆菌的细菌菌株(总计106株),在消除有机酸和过氧化氢影响的条件下,筛选其对其他细菌的拮抗活性。结果显示有5株分离株可产生细菌素,基于其宿主范围抑制和交叉抑制情况,这些细菌素各不相同。通过温度稳定性、对蛋白水解酶、脂肪水解酶和糖酵解酶的敏感性以及硫酸铵沉淀法对这些细菌素进行了初步表征。

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