Allen D G, Blinks J R, Prendergast F G
Science. 1977 Mar 11;195(4282):996-8. doi: 10.1126/science.841325.
Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10(-9) to 10(-2) molar. At very low Ca2+ concentations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.
在钙离子浓度为10⁻⁹至10⁻²摩尔的情况下,对钙敏感生物发光蛋白水母发光蛋白的发光进行了测量。在非常低的Ca²⁺浓度下,发光与钙离子浓度无关。光作为钙离子浓度函数的对数-对数图的最大斜率约为2.5。一个涉及三个钙结合位点的双态模型很好地描述了完整的关系。