Svoboda K, Schmidt C F, Schnapp B J, Block S M
Rowland Institute for Science, Cambridge, Massachusetts 02142.
Nature. 1993 Oct 21;365(6448):721-7. doi: 10.1038/365721a0.
Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.
生物马达是按规则的步幅移动吗？为了回答这个问题，我们构建了具有空间和时间敏感性的仪器，以解析分子尺度上的运动。我们使用光镊将携带单个驱动蛋白分子的二氧化硅珠沉积在微管上，并通过干涉测量法分析它们在受控负载下的运动。我们发现驱动蛋白以8纳米的步幅移动。
