使用MINFLUX在活细胞中追踪单个驱动蛋白
Tracking Single Kinesin in Live Cells Using MINFLUX.
作者信息
Deguchi Takahiro, Sergeev Nikolay Arkadievich, Ries Jonas
机构信息
European Molecular Biology Laboratory, Cell Biology and Biophysics, Heidelberg, Germany.
Max Perutz Labs, Vienna Biocenter Campus, Vienna, Austria.
出版信息
Methods Mol Biol. 2025;2881:119-131. doi: 10.1007/978-1-0716-4280-1_5.
PMID:39704940
Abstract
MINFLUX is a super-resolution fluorescence microscopy technique that enables single-molecule tracking in live cells at a single-nanometer spatial and sub-millisecond temporal resolution. This chapter describes a method for tracking fluorescently labeled human kinesin-1 in live cells using MINFLUX and analyzing kinesin stepping dynamics.
摘要
MINFLUX是一种超分辨率荧光显微镜技术，能够在活细胞中以单纳米空间分辨率和亚毫秒时间分辨率对单分子进行追踪。本章介绍了一种使用MINFLUX在活细胞中追踪荧光标记的人类驱动蛋白-1并分析驱动蛋白步移动力学的方法。
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