Ibarra-Rubio M E, Pedraza-Chaverrí J, Panduro A
Department of Nephrology and Mineral Metabolism, Instituto Nacional de la Nutrición S.Z., Tlalpan, México D.F.
Nephron. 1993;65(1):119-24. doi: 10.1159/000187452.
The messenger ribonucleic acid (mRNA) levels of albumin, fibrinogen, alpha 1-acid glycoprotein (pAGP) and transferrin were analyzed in puromycin aminonucleoside (PAN)-treated (nephrotic) and in pair-fed (PF) rats with the Northern and dot blot hybridization techniques. Albumin mRNA levels in nephrotic and PF rats were 2- and 1.5-fold higher, respectively, than in ad-libitum-fed control (C) rats 6 days after PAN treatment. On day 11, this mRNA in PAN-treated rats was 2.5-fold higher than in PF and 4-fold higher than in C rats. A differential expression at the level of specific mRNAs was also detected for fibrinogen, pAGP, and transferrin in nephrotic and PF rats. On day 6, the fibrinogen and transferrin mRNA levels were significantly higher (p < 0.05) in nephrotic than in PF rats. In contrast, pAGP mRNA levels were normal or low in nephrotic rats and increased 2-fold in PF rats. These studies indicate the differential expression of hepatic genes in nephrotic and PF rats and show that albumin gene expression is only partially regulated by diet during the nephrotic stage of the disease.
采用Northern杂交和斑点杂交技术，分析了嘌呤霉素氨基核苷（PAN）处理的（肾病）大鼠和配对喂养（PF）大鼠中白蛋白、纤维蛋白原、α1-酸性糖蛋白（pAGP）和转铁蛋白的信使核糖核酸（mRNA）水平。PAN处理6天后，肾病大鼠和PF大鼠的白蛋白mRNA水平分别比自由摄食对照（C）大鼠高2倍和1.5倍。在第11天，PAN处理大鼠的这种mRNA比PF大鼠高2.5倍，比C大鼠高4倍。在肾病大鼠和PF大鼠中，还检测到纤维蛋白原、pAGP和转铁蛋白在特定mRNA水平上的差异表达。在第6天，肾病大鼠的纤维蛋白原和转铁蛋白mRNA水平显著高于PF大鼠（p<0.05）。相反，肾病大鼠的pAGP mRNA水平正常或较低，而PF大鼠的pAGP mRNA水平增加了2倍。这些研究表明了肾病大鼠和PF大鼠肝脏基因的差异表达，并表明在疾病的肾病阶段，白蛋白基因表达仅部分受饮食调节。
