使DNA双链稳定需要多少水合作用？
How much hydration is necessary for the stabilisation of DNA-duplex?
作者信息
Maltseva T V, Agback P, Chattopadhyaya J
机构信息
Department of Bioorganic Chemistry, University of Uppsala, Sweden.
出版信息
Nucleic Acids Res. 1993 Sep 11;21(18):4246-52. doi: 10.1093/nar/21.18.4246.
Abstract
A combination of NOESY and ROESY experiments show that the higher stabilities (T(m)) of phenazine tethered matched (2) and G-A mismatched (4) DNA duplexes are due to the decrease of the exchange-rate (i.e. increase of the life-time) of the imino-protons and the reduced water activity in their minor grooves compared to their non-tethered counterparts (1) and (3).
摘要
二维核Overhauser效应光谱（NOESY）和旋转核Overhauser效应光谱（ROESY）实验结果表明，与未连接的对应物（1）和（3）相比，吩嗪连接的匹配（2）和G-A错配（4）DNA双链体具有更高的稳定性（熔解温度T(m)），这是由于亚氨基质子的交换速率降低（即寿命增加）以及小沟中水活度降低所致。
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