Loy T S, Abshier J
Department of Pathology, University of Missouri, Medical School, Columbia.
Mod Pathol. 1993 Jul;6(4):473-5.
Data has been presented suggesting that the monoclonal antibody HAM56 reacts with many adenocarcinomas but not with colonic adenocarcinomas. Previous studies have suggested that immunostaining with HAM56 may be useful in the differential diagnosis of adenocarcinoma of unknown origin. To test the specificity of HAM56 immunostaining in the diagnosis of adenocarcinomas, we studied formalin-fixed, paraffin-embedded tissue from 213 adenocarcinomas from a variety of sites using an avidin-biotin immunohistochemical technique. Positive reactivity was seen in some tumors of all types including 39% of colonic adenocarcinomas. We conclude that immunostaining with HAM56 is not specific in the differential diagnosis of adenocarcinoma of unknown origin.
已有数据表明，单克隆抗体HAM56可与许多腺癌发生反应，但不与结肠腺癌反应。先前的研究表明，用HAM56进行免疫染色可能有助于不明来源腺癌的鉴别诊断。为了检测HAM56免疫染色在腺癌诊断中的特异性，我们采用抗生物素蛋白-生物素免疫组化技术，研究了来自不同部位的213例腺癌的福尔马林固定、石蜡包埋组织。在所有类型的一些肿瘤中均观察到阳性反应，包括39%的结肠腺癌。我们得出结论，用HAM56进行免疫染色在不明来源腺癌的鉴别诊断中不具有特异性。
