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Phosphorylation of thymidylate synthase affects slow-binding inhibition by 5-fluoro-dUMP and N(4)-hydroxy-dCMP.胸苷酸合成酶的磷酸化会影响5-氟-dUMP和N(4)-羟基-dCMP的慢结合抑制作用。
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The catalytic mechanism and structure of thymidylate synthase.胸苷酸合成酶的催化机制与结构
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Kinetic scheme for thymidylate synthase from Escherichia coli: determination from measurements of ligand binding, primary and secondary isotope effects, and pre-steady-state catalysis.大肠杆菌胸苷酸合成酶的动力学机制：通过配体结合、一级和二级同位素效应以及稳态前催化的测量来确定
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Mutational analysis of the active site of indoleglycerol phosphate synthase from Escherichia coli.大肠杆菌磷酸吲哚甘油合酶活性位点的突变分析。
Protein Sci. 1998 May;7(5):1221-32. doi: 10.1002/pro.5560070518.

本文引用的文献

1
Structure-based discovery of inhibitors of thymidylate synthase.基于结构的胸苷酸合成酶抑制剂的发现
Science. 1993 Mar 5;259(5100):1445-50. doi: 10.1126/science.8451640.
2
Probing the structure and mechanism of Ras protein with an expanded genetic code.利用扩展遗传密码探究Ras蛋白的结构与机制。
Science. 1993 Feb 5;259(5096):806-9. doi: 10.1126/science.8430333.
3
Asparagine 229 in thymidylate synthase contributes to, but is not essential for, catalysis.胸苷酸合成酶中的天冬酰胺229对催化有作用，但不是催化所必需的。
Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8604-8. doi: 10.1073/pnas.90.18.8604.
4
Exclusion of 2'-deoxycytidine 5'-monophosphate by asparagine 229 of thymidylate synthase.胸苷酸合成酶的天冬酰胺229对2'-脱氧胞苷5'-单磷酸的排斥作用。
Biochemistry. 1993 Sep 14;32(36):9263-7. doi: 10.1021/bi00087a001.
5
Atomic structure of thymidylate synthase: target for rational drug design.
Science. 1987 Jan 23;235(4787):448-55. doi: 10.1126/science.3099389.
6
Saturation site-directed mutagenesis of thymidylate synthase.
J Biol Chem. 1990 Nov 5;265(31):18776-9.
7
Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate.胸苷酸合成酶与二氢尿嘧啶核苷酸（dUMP）及一种抗叶酸结合时的结构、多位点结合和片段适配
Biochemistry. 1990 Jul 31;29(30):6964-77. doi: 10.1021/bi00482a004.
8
Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2'-deoxyuridylate and 10-propargyl-5,8-dideazafolate.含有结合态5-氟-2'-脱氧尿苷酸和10-炔丙基-5,8-二氮杂叶酸的大肠杆菌胸苷酸合酶的晶体结构
J Mol Biol. 1990 Aug 20;214(4):923-36. doi: 10.1016/0022-2836(90)90346-N.
9
Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases.
Proteins. 1990;8(4):315-33. doi: 10.1002/prot.340080406.
10
Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase.胸苷酸合成酶中天冬酰胺229突变为天冬氨酸会将该酶转变为脱氧胞苷酸甲基化酶。
Biochemistry. 1992 Jun 9;31(22):5100-4. doi: 10.1021/bi00137a002.

Functional analysis suggests unexpected role for conserved active-site residue in enzyme of known structure.

作者信息

Schimmell P

机构信息

Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.

出版信息

Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9235-6. doi: 10.1073/pnas.90.20.9235.

DOI:10.1073/pnas.90.20.9235
PMID:8415683
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC47541/
Abstract
摘要