氘代蓝细菌光系统I颗粒中P700 +信号的g张量测量。
Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.
作者信息
Prisner T F, McDermott A E, Un S, Norris J R, Thurnauer M C, Griffin R G
机构信息
Francis Bitter National Magnet Laboratory, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9485-8. doi: 10.1073/pnas.90.20.9485.
Abstract
We report high-field continuous wave EPR spectra of P700+. in preparations obtained from deuterated cyanobacteria (Synechococcus lividus). Measurements were performed with photosystem I (PS-I) preparations, whole cells from cyanobacteria grown in 2H2O, and photosystem II (PS-II) preparations, as well as with protonated PS-I preparations. Because of the significantly improved resolution of our 140-GHz spectrometer (as compared with X- or Q-band EPR) the principal values of the g-tensor of the primary donor P700+. could be resolved and measured with high accuracy as g11 = 2.00304, g22 = 2.00262, and g33 = 2.00232. Other signals arising from Mn2+ and a dark signal from PS-II at g approximately 2.00266 are distinguished from the P700+. g-tensor powder pattern. The measured g values are compared with those of several bacterial reaction center donors.
摘要
我们报道了从氘代蓝细菌（蓝藻）制备物中获得的P700 +的高场连续波电子顺磁共振（EPR）光谱。使用光系统I（PS-I）制备物、在2H2O中生长的蓝细菌的全细胞、光系统II（PS-II）制备物以及质子化的PS-I制备物进行了测量。由于我们的140 GHz光谱仪的分辨率显著提高（与X波段或Q波段EPR相比），初级供体P700 +的g张量的主值得以分辨并高精度测量，结果为g11 = 2.00304、g22 = 2.00262和g33 = 2.00232。来自Mn2 +的其他信号以及在g约为2.00266处来自PS-II的暗信号与P700 +的g张量粉末图谱区分开来。将测得的g值与几种细菌反应中心供体的g值进行了比较。
相似文献
1
Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.氘代蓝细菌光系统I颗粒中P700 +信号的g张量测量。
Proc Natl Acad Sci U S A. 1993 Oct 15;90(20):9485-8. doi: 10.1073/pnas.90.20.9485.
2
Exploring the electron transfer pathways in photosystem I by high-time-resolution electron paramagnetic resonance: observation of the B-side radical pair P700(+)A1B(-) in whole cells of the deuterated green alga Chlamydomonas reinhardtii at cryogenic temperatures.通过高时间分辨率电子顺磁共振探索光系统 I 中的电子转移途径：在低温下对氘代绿藻莱茵衣藻的整个细胞中 B 侧自由基对 P700(+)A1B(-)的观察。
J Am Chem Soc. 2012 Mar 28;134(12):5563-76. doi: 10.1021/ja208806g. Epub 2012 Mar 16.
3
Structure of the P700(+ )A1(-) radical pair intermediate in photosystem I by high time resolution multifrequency electron paramagnetic resonance: analysis of quantum beat oscillations.通过高时间分辨率多频电子顺磁共振研究光系统I中P700(+)A1(-)自由基对中间体的结构：量子拍振荡分析
J Am Chem Soc. 2001 May 9;123(18):4211-22. doi: 10.1021/ja003382h.
4
Recruitment of a foreign quinone into the A(1) site of photosystem I. II. Structural and functional characterization of phylloquinone biosynthetic pathway mutants by electron paramagnetic resonance and electron-nuclear double resonance spectroscopy.一种外源醌进入光系统I的A(1)位点。II. 通过电子顺磁共振和电子-核双共振光谱对叶绿醌生物合成途径突变体的结构和功能表征。
J Biol Chem. 2000 Mar 24;275(12):8531-9. doi: 10.1074/jbc.275.12.8531.
5
Electron transfer from the acceptor A1 to the iron-sulfur centers in photosystem I as studied by transient EPR spectroscopy.通过瞬态电子顺磁共振光谱研究从受体A1到光系统I中铁硫中心的电子转移。
Biochemistry. 1994 Oct 4;33(39):11789-97. doi: 10.1021/bi00205a015.
6
Pulsed high-frequency EPR study on the location of carotenoid and chlorophyll cation radicals in photosystem II.关于光系统II中类胡萝卜素和叶绿素阳离子自由基位置的脉冲高频电子顺磁共振研究。
J Am Chem Soc. 2003 Apr 30;125(17):5005-14. doi: 10.1021/ja0295671.
7
Nanosecond electron transfer kinetics in photosystem I following substitution of quinones for vitamin K1 as studied by time resolved EPR.通过时间分辨电子顺磁共振研究了用醌类取代维生素K1后光系统I中的纳秒级电子转移动力学。
FEBS Lett. 1991 Jun 17;284(1):98-102. doi: 10.1016/0014-5793(91)80771-t.
8
Deuteration effects on the in vivo EPR spectrum of the reduced secondary photosystem I electron acceptor A1 in cyanobacteria.氘代对蓝细菌中还原态光系统I二级电子受体A1的体内电子顺磁共振谱的影响。
Biochemistry. 1999 Mar 23;38(12):3726-32. doi: 10.1021/bi982431o.
9
Contribution of vitamin K1 to the electron spin polarization in spinach photosystem I.维生素K1对菠菜光系统I中电子自旋极化的贡献。
Biochemistry. 1990 Sep 4;29(35):8030-2. doi: 10.1021/bi00487a006.
10
A1 reduction in intact cyanobacterial photosystem I particles studied by time-resolved step-scan Fourier transform infrared difference spectroscopy and isotope labeling.通过时间分辨步进扫描傅里叶变换红外差光谱和同位素标记研究完整蓝藻光系统I颗粒中的A1还原。
Biochemistry. 2005 Feb 15;44(6):1880-93. doi: 10.1021/bi0497493.
引用本文的文献
1
Cationic penetrating antioxidants switch off Mn cluster of photosystem II in situ.阳离子穿透抗氧化剂在原位关闭光系统 II 的 Mn 簇。
Photosynth Res. 2019 Nov;142(2):229-240. doi: 10.1007/s11120-019-00657-2. Epub 2019 Jul 13.
2
Selenium-induced protection of photosynthesis activity in rape (Brassica napus) seedlings subjected to cadmium stress. Fluorescence and EPR measurements.硒诱导镉胁迫下油菜（甘蓝型油菜）幼苗光合作用活性的保护。荧光和 EPR 测量。
Photosynth Res. 2010 Jul;105(1):27-37. doi: 10.1007/s11120-010-9551-y. Epub 2010 Apr 21.
3
Light-generated nuclear quantum beats: a signature of photosynthesis.光生核量子拍频：光合作用的一种特征
Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7789-93. doi: 10.1073/pnas.92.17.7789.
4
Angular orientation of the stable tyrosyl radical within photosystem II by high-field 245-GHz electron paramagnetic resonance.
Proc Natl Acad Sci U S A. 1994 Jun 7;91(12):5262-6. doi: 10.1073/pnas.91.12.5262.
本文引用的文献
1
Electron nuclear double resonance evidence supporting a monomeric nature for P700 in spinach chloroplasts.电子-核双共振证据支持菠菜叶绿体中 P700 为单体形式。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1098-101. doi: 10.1073/pnas.81.4.1098.
2
Pigment-protein interactions in the photosynthetic reaction centre from Rhodopseudomonas viridis.绿菌（Rhodopseudomonas viridis）光合作用反应中心的色素-蛋白相互作用。
EMBO J. 1986 Oct;5(10):2445-51. doi: 10.1002/j.1460-2075.1986.tb04520.x.
3
Thermodynamics and dynamics of phosphatidylcholine-cholesterol mixed model membranes in the liquid crystalline state: effects of water.液晶态下磷脂酰胆碱 - 胆固醇混合模型膜的热力学与动力学：水的影响
Biophys J. 1993 Sep;65(3):1283-94. doi: 10.1016/S0006-3495(93)81160-3.
4
Crystallization of reaction center from Rhodopseudomonas sphaeroides: preliminary characterization.球形红假单胞菌反应中心的结晶：初步表征。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4795-9. doi: 10.1073/pnas.81.15.4795.
5
Electron spin resonance of chlorophyll and the origin of signal I in photosynthesis.叶绿素的电子自旋共振与光合作用中信号I的起源
Proc Natl Acad Sci U S A. 1971 Mar;68(3):625-8. doi: 10.1073/pnas.68.3.625.
6
Exciton interaction in the photosystem I reaction center from spinach chloroplasts. Absorption and circular dichroism difference spectra.菠菜叶绿体光系统I反应中心中的激子相互作用。吸收光谱和圆二色性差光谱。
Biochemistry. 1972 Nov 21;11(24):4591-5. doi: 10.1021/bi00774a027.
7
Characterization of primary reactants in bacterial photosynthesis. I. Comparison of the light-induced EPR signal (g=2.0026) with that of a bacteriochlorophyll radical.细菌光合作用中初级反应物的表征。I. 光诱导电子顺磁共振信号（g = 2.0026）与细菌叶绿素自由基信号的比较。
Biochim Biophys Acta. 1972 May 25;267(2):363-74. doi: 10.1016/0005-2728(72)90123-5.
8
Electron spin resonance of an irradiated single crystal of L-tyrosine-HC.L-酪氨酸 - 盐酸盐辐照单晶的电子自旋共振
Proc Natl Acad Sci U S A. 1969 Feb;62(2):299-304. doi: 10.1073/pnas.62.2.299.
9
Structure of Rhodopseudomonas sphaeroides R-26 reaction center.球形红假单胞菌R-26反应中心的结构。
FEBS Lett. 1986 Sep 1;205(1):82-6. doi: 10.1016/0014-5793(86)80870-5.
10
Tyrosine radicals are involved in the photosynthetic oxygen-evolving system.酪氨酸自由基参与光合放氧系统。
Proc Natl Acad Sci U S A. 1987 Oct;84(20):7099-103. doi: 10.1073/pnas.84.20.7099.
Zotero 插件